Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Geomicrobiological testing
Patent
1987-12-17
1991-10-08
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Geomicrobiological testing
435 6, 435 34, 435 35, 435 8, C12Q 164
Patent
active
050553976
ABSTRACT:
Geomicrobiological exploration method employing one or more culture, luminometry or tritiated thymidine assays of microbes (bacteria) for sensitivity, determined as survival rate in percentage, of the microbes to selected toxic materials, preferably heavy metals and/or hydrocarbons. The survival assay values are plotted as contours on a geophysical map and target areas of potential interest are identified and further evaluated by inspection or other techniques. Examples show actual use of the techniques to identify a target area in a raw prospect petroleum lease area which was drilled to successful oil discovery thus proving the method. Sensitivity test incubation ranges from 1-3 hours (preferably 2 hours) for heavy metals at 20-25 degrees C., and 3-10 min. for pentene/hexane at 15-20 degrees C. Luminometry is fastest, being run in 1.5-30 minutes and most suitable for field surveys. Toxics concentrations may range from 0.001 ug/ml to 15,000 ug/ml for heavy metals to 0.001-25 vol % for hydrocarbons.
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Michaels Glenda B.
Riese Walter C.
Atlantic Richfield Company
Dulin Jacques M.
Rosen Sam
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