Gentamicin-resistance genes and their use as markers

Details Gentamicin-resistance genes and their use as markers Gentamicin-resistance genes and their use as markers

1987-04-30

1990-04-17

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

536 27, 435320, 4352523, 43525235, 435886, 935 29, 935 56, C12N 120, C12N 700, C12P 2100, C12R 1465

Patent

active

049180150

ABSTRACT:
S. ghanaensis DSM 2932 is resistant to gentamicin at up to 20 .mu.g/ml. Total digestion of the genomic DNA with BglII, incorporation of the restriction fragments into a suitable plasmid, and selection using gentamicin results in gentamicin-resistant clones which contain a 7 kb fragment with the gentamicin-resistance gene. The plasmid pPH1JI likewise contains a gentamicin-resistance gene located on a 2.3 kb HindIII-BamHI fragment. These genes are suitable as markers, in particular for Streptomycetes vectors.

REFERENCES:
patent: 4332900 (1982-06-01), Manis et al.
Hirsch & Beringer, A Physical Map of pPH1JI and pJB4JI, Plasmid 12, 139-141 (1984).
Vieira & Messing, the pUC Plasmids an M13mp 7-Derived System for Insertion Mutagenesis and Sequencing with Synthetic Universal Primers, Gene, 19 pp. 259-268 (1982).
Yangsch-Perron et al., Improved M13 phage Cloning Vectors and Host Strains: Nucleotide Sequences of the M13mp18 and pUC19 Vectors, Gene, 33 (1985), 103-119.

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