Genomic DNA fragment of Streptococcus pneumoniae, hybridization

Details Genomic DNA fragment of Streptococcus pneumoniae, hybridization Genomic DNA fragment of Streptococcus pneumoniae, hybridization

1995-06-07

1998-06-23

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 2432, C12Q 168

Patent

active

057703620

ABSTRACT:
The invention relates to a fragment of the genomic DNA of Strepeococcus pneumoniae, a probe capable of specifically hybridizing with the genomic DNA of Streptococcus pneumoniae, a specific primer for the amplification, by polymerization, of the genomic DNA, a reagent and a method which are used with the probe and, optionally, the primer, for specifically detecting Streptococcus pneumoniae in a biological sample. The probe of the invention is a nucleotide sequence having at least 70% homology with at least a portion of a consensus sequence of the genomic DNA of Streptococcus pneumoniae, this consensus sequence being chosen from the nucleotide sequences SEQ ID NO 2, SEQ ID NO 3, SEQ ID NO 4, which are identified in the description, and their respective complementary sequences.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
Fennol et al., European J. Microbiol. Infect. Diseases, 9:396-410, Jun. 1990.
Prudhomme et al., J. Bacteriol., 171(10:5332-5338, Oct. 1989.
Radnis et al., "Genetic Transformation Streptoccus Pneumoniae. . . Sequence of recP," Journal of Bacteriology, vol. 172, No. 7, Jul. 1990, pp. 3669-3674.
Fenoll et al., "Identification of Atypical Strains of Streptococcus Pneumoniae by a Specific DNA probe,"European Journal of Clinical Microbiology and Infectious Diseases, vol. 9, Jun. 1990, pp. 396-401.
Prudhomme, M. et al., "Nucleotide Sequence of the Streptococcus Pneumoniae hexB Mismatch Repair Gene: Homology of HexB to MutL of Salmonella typhimurium and to PMS1 of Saccharomyces cerevisae," J. of Bacteriology, vol. 10, Oct. 1989, pp. 5332-5338.
Alloing, G., et al., "The ami locus of the Gram-positive bacterium Streptococcus pneumoniae is a similar to binding protein-dependent transport operons of Gram-negative bacteria," Molelcular Microbiology, (1990),4(4), 633-644.
Sanger, F., et al., DNA sequencing with chain-terminating inhibitors (DNA polymerase
ucleotide sequences/bacteriophage .phi..times.174), Proc. Natl. Acad. Sci. USA, vol. 74, No. 12, pp. 5436-5467, Dec. 1977 Biochemistry.
Lefevre, J., et al., "Donor Deoxyribonucleic Acid Length and Marker Effect in Pneumococcal Transformation," J. of Bacteriology, Apr. 1979, pp. 80-86, vol. 138, No. 1.
Tiraby, G., et al., "Marker Discrimination in Deoxyribonucleic Acid-Mediated Transformation of Various Pneumococcus Strains," J. of Bacteriology, vol. 121, No. 2, Feb. 1975, pp. 608-618.
Cokendall, Alan L., "Classification and Indentification of the Viridans Streptococci, " Clinical Microbiology Reviews, vol. 2, No. 3, Jul. 1989, pp. 315-328.
Kilian, M. et al., "Taxonomic Study of Viridans Streptococci: Description of Streptococcus gordonii sp. nov. and Emended Descriptions of Streptococcus sanguis (White and Niven 1946), Streptococcus oralis (Bridge and Sneath 1982), and Streptococcus mitis (Andrews and Horder 1906)," Intl. J. of Systematic Bacteriology, vol. 39, No. 4, Oct. 1989, pp. 471-484.
Prere, Marie-Francoise et al., "DNA methylase activities in a Neisseria gonorrhoeae extract (In vitro DNA methylation; Neisseria gonorrhoeae; gonococcal DNA modification systems)," FEMS Microbiology Letters 33, (1986) 37-41.
Haisman, R., et al., "Mutants of Streptococcus gordonii Challis over-producing glucosyltransferase," J. of General Microbiology, .(1991), 137, 483-489.
Kilpper-Balz, R., et al., "Molecular Relationships and Classification of Some Viridans Streptococci as Streptococcus oralis and Emended Description of Streptococcus oralis (Bridge and Sneath 1982)," International Journal of Systematic Bacteriology, vol. 35, No. 4, Oct. 1985, pp. 482-488.
Lewin, Science 237:1570, 1987.
Reeck et al., Cell 50:667, Aug. 28, 1987.

Affiliated with

Also associated with

Rating

Genomic DNA fragment of Streptococcus pneumoniae, hybridization does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Genomic DNA fragment of Streptococcus pneumoniae, hybridization , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Genomic DNA fragment of Streptococcus pneumoniae, hybridization will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1393358