Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-06-06
1998-03-03
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
530370, 530379, 435 691, 4353201, 435 692, 536 232, 536 236, C12N 922, C07K 14415
Patent
active
057233268
ABSTRACT:
The present inventors discovered a novel genome coding Phytolacca insularis antiviral protein(PIP) isolated from Phytolacca insularis Nakai; and developed a recombinant vector for said PIP genome expression and a microorganism transformed therewith. PIP genome of the present invention has nucleotide homology of about 82%, compared with the genome of Phytolacca americana antiviral protein isolated from Phytolacca americana L., which is closely related to the Phytolacca insularis Nakai. PIP cDNA is consist of 918 bp of one open reading frame and termination codon; and polyadenylation signal which is ubiquitous in mRNA of most plants and animals, appears to be located in the upstream of 33 bp from the polyadenylation site. Recombinant PIP of the invention was proved to inhibit the growth of E. coli HB101 transformed with said expression vector.
REFERENCES:
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Choi Kyu-Whan
Jeon Hong-Seob
Kim Man-Keun
Lee Kwan-Ho
Moon Young-Ho
Jinro Limited
Prouty Rebecca
Wax Robert A.
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