Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1999-01-21
2000-07-25
Park, Hankyel
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 701, 435414, 435419, 435425, 435267, C12P 2106, C12P 2104, C12N 504, C12N 502, C07G 1700
Patent
active
060935545
ABSTRACT:
The invention relates to genetic manipulations of eukaryotic organisms, with recombinant DNA comprising RNA virus derived sequences for protecting such organisms against RNA viruses or enabling inducible or tissue-specific production of foreign proteins/peptides or RNAs. One embodiment of the recombinant DNA according to the invention comprises recombinant DNA, comprising two, 12-250 base pair long, inverted repeat nucleotide sequences with therebetween at least one nucleotide sequence which is derived from RNA virus which for its replication is dependent upon a viral RNA/RNA polymerase, said RNA virus derived sequence comprising at least cis elements for replication but no gene that codes for viral RNA/RNA polymerase and no gene that codes for viral coat protein. The invention also relates to eukaryotic or prokaryotic cells or organisms which incorporate the recombinant DNA according to the invention. Further, the invention relates to a method of protecting such cells or organisms by genetically incorporating recombinant DNA according to the invention.
REFERENCES:
Robert C. King, William D. Stansfield, "A Dictionary of Genetics," Third Edition, Oxford University Press, p. 336 (1985).
Virginia Walbot and George Bruening, "Plant Development and Ribozymes for Pathogens," Nature, vol. 334, pp. 196-197 (1988).
Eli Gilboa, Martin A. Eglitis, Philip W. Kantoff and V. French Anderson, "Transfer and Expression of Cloned Genes Using Retroviral Vectors," Bio Techniques, vol. 4, No. 6 (1986).
John Van Emmelo, Paul Ameloot and Walter Friers, "Expression in Plants of the Cloned Satellite Tobacco Necrosis Virus Genom and of Derived Insertion Mutants," Virology, vol. 157, pp. 480-487 (1987).
Ameloot Paul
De Lafonteyne Jean
Fiers Walter
Haute Eddie Van
Aveve N.V.
Matton Clovis
Park Hankyel
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