Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Plant cell or cell line – per se – contains exogenous or...
Reexamination Certificate
2000-03-16
2003-03-18
McElwain, Elizabeth F. (Department: 1638)
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Plant cell or cell line, per se, contains exogenous or...
C435S320100, C536S023600
Reexamination Certificate
active
06534313
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates generally to plant growth and development and more specifically to methods of modifying brassinolide activity in plants by modulating cytochrome P450 activity.
BACKGROUND OF THE INVENTION
A plant is considered healthy when it can carry out its physiological functions, such as cell division, differentiation, development, photosynthesis, absorption and translocation of water and nutrients from the soil, metabolism, reproduction, and storage of food supplies, without disruption. When plant functions are disturbed by pathogens or insects, the plants become diseased or destroyed. Disease can be defined as the malfunctioning of plant host cells and tissues caused by continuous irritation by a pathogenic agent or insect. A disease involves abnormal changes in the form, physiology, or behavior of the plant.
Representative insects or pests that attack plants include Coleoptera and Lepidoptera such as western corn root worm (Diabrotica virgifera virgifera), northern corn root worm (Diabrotica longicornis barberi), southern corn rootworm (Diabrotica undecimpunctata howardi), cotton bollworm, European corn borer, corn root webworm, pink bollworm and tobacco budworrn. The transgenic plants are preferably monocotyledoneous or dicotyledoneous plants. Plant pathogenic bacteria also cause a variety of plant disease symptoms. About 80 species of bacteria (e.g.,
Pseudomonas viridiflava, Xanthomonas campestris
pv. asclepiadas,
Xyella fastidiosa, Acidovorax albilineans
, and
Acidovorax avenae
sspl citrulli) cause disease in plants, including fruit rot, galls, wilts, blight, and leaf spots. As bacteria multiply quickly, controlling them early in the disease process is critical. Copper and streptomycin compounds are the only chemical compounds currently available for the control of bacterial diseases.
Genetic engineering of plants, which entails the isolation and manipulation of genetic material, e.g., DNA or RNA, and the subsequent introduction of that material into a plant or plant cells, has changed plant breeding and agriculture considerably over recent years. Increased crop food values, higher yields, feed value, reduced production costs, pest resistance, stress tolerance, drought resistance, the production of pharmaceuticals, chemicals and biological molecules as well as other beneficial traits are all potentially achievable through genetic engineering techniques. Genetic engineering techniques supplying the genes involved in pathogen resistance have the potential to substantially affect crop production.
Traditionally, the control of plant stature has been through the process of selective breeding. Often dwarf plants are chosen for their ornamental value or their improved ability to survive under mechanical stress, such as high wind. However, this breeding process can take many years. An alternative way to rapidly create dwarf plants is by the exogenous application of certain organic compounds, such as the gibberellin biosynthesis inhibitor, uniconazole. However, these compounds are expensive and must be applied throughout the plant life cycle.
Light also has an important role in plant development, both for photosynthesis and as a developmental cue. A variety of photoreceptors respond to the quality, quantity, direction and duration of the light environment. In Arabidopsis there are five red/far-red absorbing phytochromes (phyA-phyE), two blue/UVA absorbing cryptochromes (cry1 and 2) and the less understood UVB photoreceptors. All affect gross morphological changes in seedling development as they deetiolate, making the transition from growth in the dark to growth in the light (C. Fankhauser and J. Chory,
Annu Rev Cell Dev Biol
13:203-29, 1997). Genetic analysis demonstrates a complex web of interactions between these photoreceptor signaling pathways (Casal and Mazzella,
Plant Physiol
118:19-25, 1998; M. M. Neff and J. Chory,
Plant Physiol.
118:27-35, 1998; L. Hennig et al.,
Planta
208:257-263, 1999; G. Lasceve et al.,
Plant Physiol
120:605-614, 1999). There is also a distinct class of photoreceptors, the phototropins (e.g. NPH1), which effect the directional growth of seedlings (E. Liscum and W. R. Briggs,
Plant Cell
7:473-485, 1995; E. Huala et al.,
Science
278:2120-2123, 1997; J. M. Christie et al.,
Science
282:1698-701, 1998), a process that can be modified by the activity of phytochromes (B. M. Parks et al.,
Plant Physiol
. 110:155-162, 1996).
Plant hormones can also contribute to photomorphogenic responses. Some photomorphogenetic mutants resemble mutants involved in phytohormone biosynthesis or sensing. For example, the GA signaling mutant spindly resembles plants with mutations in phyB, which have long stems, pale leaves and early flowering. This phenotype can also be mimicked in wild type plants by the application of GA
3
(S. E. Jacobsen and N. E. Olszewski,
Plant Cell
5:887-896, 1993). Genetic analysis of GA and phytochrome mutants points to interactions between these two signal transduction systems for certain responses (J.Chory,
Plant Cell
9:1225-34, 1997). However, other responses, such as flowering, are likely to be independently controlled by both systems (M. A. Blázquez and D. Weigel,
Plant Phys
120:1025-32, 1999).
Gibberellins are not the only hormones that are involved in light signaling. Auxins clearly have some role in photomorphogenesis. For example, auxin transport is affected in a light dependent manner (P. J. Jensen et al.,
Plant Physiol
116:455-462, 1998). Genetic analysis also points towards a role for auxin in light signal transduction. The shy2 mutation, identified in a suppressor screen for mutants with reduced levels of all phytochromes (Kim et al.[In Process Citation].
Plant J
15:61-68) or in a null mutant of phyB (J. W. Reed et al.,
Genetics
148:1295-1310, 1998), resides in the auxin induced gene IAA3 (Q. Tian and J. W. Reed,
Development
126:711-21, 1999). A third example of the interplay between photomorphogenesis and phytohormones is that many brassinosteroid mutants have been identified in genetic screens for plants that can undergo deetiolation in the absence of a light cue (for review see (J. Li and J. Chory,
Exp Bot
50:275-282, 1999)). When these mutants are grown in the dark, their seedlings have short hypocotyls with cotyledons that begin to develop as if growing in the light. As adults, these mutants are dwarfs with dark-green, epinastic leaves and short stems and petioles. They are slow growing with delayed senescence. Each of these adult phenotypes is essentially the opposite of mutants lacking phytochrome B (for review see (Chory, 1997, supra)).
Genetic screens for loss-of-function mutations have led to the identification of many loci thought to be involved in photomorphogenesis (Fankhauser and Chory, supra). However, these screens may miss important components of light signal transduction that are either redundant members of a gene family or are essential for survival. The role of such genes may only be identified in gain-of-function mutant screens. One method for targeting gain-of-function mutations is through extragenic suppressor analysis (G. Prelich,
Trends Genet
15:261-6, 1999).
This approach has been used successfully in Arabidopsis to identify dominant or semidominant mutants involved in light signal transduction (A. Pepper and J. Chory,
Genetics
145:1125-37, 1997; Kim et al., supra; Reed et al., 1998, supra). However, positional cloning of dominant or semidominant extragenic suppressors can be difficult and time consuming if they do not have a phenotype in a wild type background. shy2 is the only dominant, extragenic suppressor mutation cloned by map based methods in Arabidopsis and has a striking phenotype in a wild type background (Tian and Reed, supra).
A gene tagging approach has been used to circumvent the difficulties of map-based cloning of mutations in Arabidopsis. In this approach, mutants are generated by transformation with Agrobacterium mediated transfer-DNA (T-DNA). Since the T-DNA sequence is known, mutations that are tagge
Chory Joanne
Neff Michael M.
Collins Cynthia
Knobbe Martens Olson & Bear LLP
McElwain Elizabeth F.
The Salk Institute for Biological Studies
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