Genetically enhanced cellulase production in Pseudomonas cellulo

Details Genetically enhanced cellulase production in Pseudomonas cellulo Genetically enhanced cellulase production in Pseudomonas cellulo

1997-09-22

1999-09-28

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435209, 43525234, 435262, 435263, 424 9461, C12P 1914, C12N 942

Patent

active

059587407

ABSTRACT:
An enhanced strain of Pseudomonas celllulosa was obtained by introducing a recombinant genetic construct comprising a heterologous cellulase gene operably connected to a promoter into ATCC 55702, mutagenizing the transformants by treatment with MNNG, and selecting a high cellulase producing transformant. The transformant, designated Pseudomonas cellulosa ATCC XXXX, exhibits enhanced levels of cellulase production relative to the untransformed Pseudomonas cellulosa strain #142 ATCC 55702.

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patent: 5702940 (1997-12-01), Dees
Svheirlinck, T. et al. "Cloning and expression of cellulase and xylanase genes in Lactobacillus plantarum." Applied Microbiology and Biotechnology (1990), vol. 33, pp. 534-541.
Lejeune, A. et al. "Cloning of an endoglucanase gene from Pseudomonas fluorescens var. cellulosa into Escherichia coli and Pseudomonas fluorescens" Journal of Industrial Microbiology (Jun. 1986), vol. 1, No. 2, pp. 79-86.

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