Genetically engineered reporter bacteria for the detection of ba

Details Genetically engineered reporter bacteria for the detection of ba Genetically engineered reporter bacteria for the detection of ba

1995-12-19

1998-03-03

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 5, 435 6, 435 8, 435 29, 435 691, 435 698, C12N 120

Patent

active

057233306

ABSTRACT:
A method for testing for target bacteria involves adding bacteriophage to a sample to infect the bacteria in the sample; killing extracellular bacteriophage without killing phage-infected bacteria; amplifying bacteriophage remaining in the sample; and causing the bacteriophage to infect reporter bacteria and thereby produce an observable signal. The reporter bacteria are genetically engineered to have an indicator gene which on expression gives rise to a detectable signal, wherein expression of the indicator gene is initiated on bacteriophage infection of the bacteria.

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D. Glover, Principles of Gene Cloning, pp. 1-20 (1984).
M. Boylan et al., J. Biol. Chem., 264(4), 1915-1918 (1989).
Hirotani et al., FEMS Microbiology Letters, 77, 13-18 (1991).
Goliger et al, "Bacteriophage 82 Gene Q and Q Protein" Journal of Biological Chemistry vol. 262:24 pp. 11721-11725 Aug. 25, 1987.
Boylan et al. "Fused Bacterial Luciferase . . . " Journal of Biological Chemistry vol. 264: 4 pp. 1915-1918 Feb. 5, 1989.
Miyamoto et al. "Organization of the Lux Structural Genes . . . " Journal of Biological Chemistry vol. 263: 26 pp. 13393-13399 Sep. 15, 1988.

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