Genetically engineered plasmid and organisms for the production

Details Genetically engineered plasmid and organisms for the production Genetically engineered plasmid and organisms for the production

1988-09-22

1991-10-15

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435255, 4353201, 536 27, C12P 764

Patent

active

050574191

ABSTRACT:
A DNA fragment containing a gene encoding a fatty acid desaturase enzyme is isolated. The gene is then employed by introducing it into other cells to alter or enhance the production of oils in such cells, alternatively the gene or nucleic acid sequences of the gene can be employed to isolate other genes encoding for related, but different desaturase enzymes.

REFERENCES:
patent: 4661454 (1987-04-01), Botstein et al.
patent: 4771002 (1988-09-01), Gelvin et al.
Thiede et al., The Journal of Biological Chemistry, vol. 261, No. 28, Oct. 5, pp. 13230-13235 Stearyl Coenzyme Adesatinase.
"The OLE 1 Gene of Saccharomyces cerevisiae Encodes the .DELTA.9 Fatty Acid Desaturase and Can Be Functionally Replaced by the Rat Stearoyl-CoA Desaturase Gene," Stukey, J. E. et al., J. Biol. chem. 265:20144-149 (1990).
"Nile Red: A Selective Fluoroscent Stain for Intracellular Lipid Droplets", Greenspan, P. et al., J. Cell Biol. 100:965-973 (1985).
"Spectrofluorometric Studies of the Lipid Probe Nile Red", Greenspan, P. and S. D. Fowler, J. Lipid Research 26:781-789 (1985).
"Enzyme Nomenclature", Commission on Biochemical Nomenclature, Academic Press, pp. 127, 550 (1979).
"Biological Research on Industrial Yeast Vol. I", Stewart, G. G. et al., eds., CRC Press, p. 2 (1987).
"Microbiology", A. I. Braude ed. W. b. Saunders Co., pp. 134-35 (1982).
"Bacterial Synthesis of Active Rat Stearl-CoA Desaturase Lacking the 26-Residue Amino-Terminal Amino Acid Sequence", Strittmatter, P. et al., J. Biol. Chem., 263:(5):2532-35 (1988).
"Isolation and Characterization of OLCl, a Gene Affecting Fatty Acid Desaturation from Saccharomyces cerevisiase Stuckey", J. E. et al., J. Biol. Chem., 264(28):16537-16544 (1989).
"Nutritional Regulation of Yeast -9 Fatty Acid Desaturase Activity", Bossie, M. and C. E. Martin, J. Bacteriol. 171(12): 6409-6413 (1989).

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