Genetically engineered modification of potato to obtain amylopec

Details Genetically engineered modification of potato to obtain amylopec Genetically engineered modification of potato to obtain amylopec

1995-06-06

1998-10-20

Wilson, James O.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 45, 536 46, 536 553, 536124, 536127, 800200, 800205, C07H 106, C07H 108

Patent

active

058247980

ABSTRACT:
Genetically engineered modification of potato for suppressing formation of amylose-type starch is described.
Three fragments for insertion in the antisense direction into the potato genome are also described. Moreover, antisense constructs, genes and vectors comprising said antisense fragments are described. Further a promoter for the gene coding for formation of granule-bound starch synthase and also the gene itself are described.
Also cells, plants, tubers, microtubers and seeds of potato comprising said antisense construct are described.
Finally, amylopectin-type starch, both native and derivatized, derived from the potato that is modified in a genetically engineered manner, as well as a method of suppressing amylose formation in potato are described.

REFERENCES:
patent: 4723052 (1988-02-01), Cochran
patent: 5349123 (1994-09-01), Shewmaker et al.
"Inhibition of the Expression of the Gene for Granule-Bound Starch Synthase in Potato by Antisense Constructs", R.G.F. Visser et al., Mol Gen Genet, vol. 225, pp. 289-296, (1991).
"Molecular Cloning and Partial Characterization of the Gene for Granule-Bound Starch Synthase from a Wildtype and an Amylose-Free Potato (Solanum Tuberosum L.0", R.G.F. Visser et al., Plant Science, vol. 64, (1989), pp. 185-192.
"Structural and Functional Analysis of Two Waxy Gene Promoters from Potato", W. Rohde et al., J. Genet. & Breed. 44:311-315, (1990).
Isolation of an Amylose-Free Starch Mutant of the Potato (Solanum Tuberosum L.), J.H.M. Hovenkamp-Hermelink et al., Theoretical and Applied Genetics, (1987) 75, pp. 217-221.
"Sequence of the Structural Gene for Granule-Bound Starch Synthase of Potato (Solanum Tuberosum L.) and Evidence for a Single Point Deletion in the amf Allele", Feike R. van der Leij et al. Mol Gen Genet, vol. 228, pp. 240-248, (1991).
"Manipulation of the Starch Composition of (Solanum Tuberosum L.) Using Agrobacterium Rhizogenes Mediated Transformation", Ph D. Thesis of Richard G.F. Visser, Rijkduniversiteit Groningen, Feb. 27, 1989, pp. 1-141.
"Evidence that the waxy protein of pea (Pisum sativum L.) is Not the Major Starch-Granule-Bound Starch Synthase", Alison M. Smith, Planta, (1990), vol. 182, pp. 599-604.
"Characterization of cDNAs Encoding Two Isoforms of Granule-Bound Starch Synthase Which Show Different Expression in Developing Storage Organs of Pea and Potato", Ian Dry et al., The Plant Journal, (1992), 2(2), pp. 193-202.
Hovenkamp-Hermelink et al., Theoretical and Applied Genetics, vol. 75 pp. 217-221 (1987).
Hovenkamp-Hermelink et al., Potato Research, vol. 31, pp. 241-246, (1988).

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