Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1995-06-06
1999-01-05
Wilson, James O.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 236, 536 245, 536102, 800205, C08B 3300
Patent
active
058564675
ABSTRACT:
Genetically engineered modification of potato for suppressing formation of amylopectin-type starch is described.
The invention describes an antisense construct for inhibiting, to a varying extent, the expression of the gene coding for formation of branching enzyme (BE gene) in potato, said antisense construct comprising a tuber-specific promoter, transcription start and the first exon of the BE gene, inserted in the antisense direction.
Also cells, plants, tubers, microtubers and seeds of potato comprising said antisense construct are described.
Finally, amylose-type starch, both native and derivatized, derived from the potato that is modified in a genetically engineered manner, as well as a method of suppressing amylopectin formation in potato are described.
REFERENCES:
patent: 4723052 (1988-02-01), Cochran
patent: 5047373 (1991-09-01), Joyce
"Isolation of a Q-Enzyme with M, 103 000 From Potato Tubers", Phytochemistry, vol. 30, No. 2, pp. 437-444, (1991), Andreas Blennow and Gote Johansson.
"Immunological Comparison of the Starch Branching Enzymes from Potato Tubers and Maize Kernels", Plant Physiology, vol. 90, (1989), pp. 75-84, Greetje H. Vos-Scheperkeuter, et al.
"Structural and Functional Analysis of Two Waxy Gene Promoters from Potato", W. Rohde et al., J. Genet. & Breed. 44:311-315, (1990).
"Antisense RNA inhibition of Polygalacturonase Gene Expression in Transgenic Tomatoes", C.J.S. Smith et al., Nature, Letters to Nature, vol. 334, Aug. 25, 1988, pp. 724-726.
"Organ-Specific Gene Expression in Potato: Isolation and Characterization of Tuber-Specific cDNA Sequences", S. Rosahl et al., Molecular Gen Genet, (1986) 202: pp. 368-373.
"Cloning and Expression Analysis of a Potato cDNA that Encodes Branching Enzyme: Evidence for Co-expression of Starch Biosynthetic Genes", Jens Kossmann et al., Molecular Gen Genet, (1991) 230: pp. 39-44.
Hovenkamp-Hemelink et al., Theoretical and Applied Genetics, vol. 75, pp. 217-221, (1987).
Hofvander Per
Persson Per T.
Tallberg Anneli
Wikstrom Olle
Amylogene HB
Wilson James O.
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