Genetically engineered microorganisms for massive production of

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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4351723, 435202, 435317, 435849, 435839, 935 14, 935 29, 935 31, 935 73, 935 74, C12N 1500, C12N 120, C12N 928, C12R 119

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044697910

ABSTRACT:
Genetically engineered microorganisms are provided which contain recombinant DNA with an amylase coding gene. Improved yields of amylase enzymes are obtained by cultivating these microorganisms.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
Yoneda et al., Cloning of a Foreign Gene Coding for .alpha. Amylase in Bacillus subtilis Biochem Biophy Res. Com., vol. 91, 1979, pp. 1556-1564.
Shinomiya et al., Isolation of a Bacillus subtilis Transforment Producing Thermostable .alpha.-Amylase by DNA from a Thermophilic Bacteum Biochem Bioph. Res. Com., vol. 96, 1980, pp. 175-179.
Broda Plasmids 1979, Freeman and Co., San Francisco, pp. 5-23.
Lewin Gene Expression, 1977, John Wiley & Sons, New York, p. 269.
Timmis, et al., DNA Cloning and the Analysis of Plasmid Structure and Function, Prog. Molec. Subcellular Biol. 6, 1978, pp. 1-58.
Murray et al., Lamboid Phages that Simplify the Recovery of in vitro Recombinants, Molec. Gen. Genet, 150, 1977, pp. 53-61.
Hohn, Packaging Recombinant DNA Molecules into Bacteriophage Particle in vitro, Proc. Natl. Acad. Sci. USA, 74, 1977, pp. 3259-3263.
Nomura, et al., Construction of Transducing Phage p. 11, Containing Alpha-Amylase Structural Gene of Bacillus subtilis, Ag. Bio. Chem. 43,2637-43,2638, 79.
Yoneda, Increased Production of Extracellular Enzymes, Applied and Environmental Microbiology, 39, 274-276, 1980.
MacDonald, et al., Nature, Structure of a Family of Rat Amylase Genes, 287,117-287,122, (1980).

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