Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1998-09-28
2000-02-29
Nutter, Nathan M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435136, 435146, 435155, 435156, 4352528, 4353171, 4353201, 435849, C12P 700, C12P 702, C12P 722, C12P 740, C12P 742
Patent
active
060308192
ABSTRACT:
The present invention pertains to a method for economical biofermentative production of 4-hydroxybenzoic acid (PHB) using genetically engineered E. coli. According to the invention, a plasmid is provided which controls the overexpression of chorismate pyruvate lyase, the bacterial enzyme which catalyzes the production of PHB from chorismate. Mutant E. coli selected with a unique two-step screening assay to overproduce chorismate have been transformed with this plasmid, providing a biocatalyst that efficiently converts glucose to PHB.
REFERENCES:
patent: 4681852 (1987-07-01), Tribe
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"Toward a Science of Metabolic Engineering", by James E. Bailey, Science 252, pp. 1668-1675 (1991).
"Microbial Production of Specifically Ring. 13C-Labelled 4-Hydroxybenzoic Acid", by R. Muller et al., Appl. Microbiol. Biotechnol. 43: pp. 985-988 (1995).
"Genetic Engineering of Metabolic Pathways Applied to the Production of Phenylalanine", by K. Backman et al., Annals New York Academy of Sciences 589, pp. 16-24 (1990).
"Formation of 4-Hydroxybenzoate in Escherichia Coli: Characterization of the ubiC Gene and its Encoded Enzyme Chorismate Pyruvate-lyase", by M. Siebert et al., Microbiology 140: pp. 897-904 (1994).
"Cloning and Sequencing of Escherichia Coli ubiC and Purification of Chorismate Lyase", by Brian P. Nichols et al., Journal of Bacteriology 174 (16): pp. 5309-5316 (1992).
Amaratunga Mohan
Johnson Bruce Fletcher
Lobos John Henry
Williams Eric Douglas
General Electric Company
Johnson Noreen C.
Nutter Nathan M.
Stoner Douglas E.
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