Genetically engineered eucaryotic host cells capable of expressi

Details Genetically engineered eucaryotic host cells capable of expressi Genetically engineered eucaryotic host cells capable of expressi

1990-07-30

1991-03-26

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4352401, 4352402, 435235, 4353201, 536 27, 935 34, 935 37, 935 66, 935 68, 935 69, 935 70, 935 71, C12N 700, C12N 1500

Patent

active

050028740

ABSTRACT:
Eucaryotic host cells are disclosed which contain a DNA molecule encoding an eIF-2.alpha. mutant and preferably a DNA sequence encoding a desired heterologous protein. The DNA sequences are linked to expression control sequences permitting expression of the mutant eIF-2.alpha. gene and the heterologous gene. Culturing such cells provides a method for the production of the desired heterologous protein. The mutations eliminate one or both serine residues at positions 48 and 51 of the eIF-2 sequence. In another aspect of the invention, the eIF-2 5'-untranslated sequence was observed to have effects on translation of heterologous mRNAs.

REFERENCES:
Wittenhall, R. E. H. et al., 1986 (Sep. 25), J. Biol. Chem. 261(27), 12444-12447.
Morinager, Y. et al., 1984, Bio/Technology 2, 636-639.
Colhurst, P. R. et al., Jul. 1987, Eur. J. Biochem. 166:357-363.
Kudlicki, W. et al., 1987, FEBS Letter 215(1), 16-20 (May 1987).
Pathak, U. K. et al., Feb. 1988, Mol. Cell Biol 8(2), 993-995.
Ernst, H. et al., Jan. 25, 1987, J. Biol. Chem. 262(3), 1206-1212.

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