Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1992-06-18
1995-05-23
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 8, 435 691, 435 697, 435 698, 435 701, 4351723, 435207, 4352351, 4352402, 4353201, 536 221, 536 234, 536 2372, 536 241, C12Q 170, C12Q 166, C12P 2106, C12N 500
Patent
active
054181321
ABSTRACT:
A diagnostic assay for detecting the presence of an infectious herpes virus in a specimen and a genetically engineered cell line for use in such assay are disclosed. The cell line used in the assay expresses a reporter gene only if infectious herpes virus is present in the specimen. The assay involves inoculating a DNA-transfected cell line with a specimen suspected of containing a herpes virus, allowing a sufficient period of time for the herpes virus infectious cycle to proceed, and detecting and quantifying the number of herpes virus-infected cells to determine the number of infectious herpes virus virions in the specimen. The cell line is a DNA-transfected cell line susceptible to infection by a herpes virus which is stably transformed with a chimeric gene comprising a herpes virus inducible promoter and a gene coding for an enzyme, the expression of the enzyme being dependent upon and quantitatively proportional to the presence of herpes virus. A kit for such assay is also provided.
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Kim Hyosuk
Washington University
Wax Robert A.
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