Genetically engineered cDNA of rat bcl-x gene and an...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

Reexamination Certificate

active

07626003

ABSTRACT:
The present invention provides an genetically engineered cDNA of the rat bcl-x gene, which has at least one substitution selected from the substitutions that change residues 22 Tyr to Phe, residues 26 Gln to Asn and residues 165 Arg to Lys, in the coding region of rat bcl-x cDNA of SEQ ID NO: 1, a recombinant vector containing the engineered cDNA, a cell into which the recombinant vector was introduced, and an improved protein of Bcl-xL. The improved protein of Bcl-xLis useful as an ingredient for remedies for various diseases accompanied with cell death since it effectively inhibit cell death such as apoptosis.

REFERENCES:
Mullins, Embo J., vol. 8, pp. 4065-4072, 1989.
Taurog, J. Immunol., vol. 141, pp. 4020-4023, 1988.
Mullins, J. Clin. Invest., vol. 98, pp. S37-S40, 1996.
Niemann, Transg. Res., vol. 7, pp. 73-75, 1997.
Cameron, Molec. Biol., vol. 7, pp. 253-265, 1997.
Mullins, Hypertension, vol. 22, pp. 630-633, 1993.
Mullins, Nature, vol. 344, pp. 541-544, 1990.
Hammer, Cell, vol. 63, pp. 1099-1112, 1990.
Overbeek, “Factors affecting transgenic animal production”, Transgenic Animal Technology, pp. 86-98, 1994.
Wall, Theriogenology, vol. 45, pp. 57-68, 1996.
Houdebine, J. Biotech., vol. 34, pp. 269-287, 1994.
Human Gene Therapy, vol. 7, pp. 1781-1790, 1996.
Orkin and Motulsky, NIH ad hoc committee Dec. 1995—http:/www.nih.gov
ews/panelrep.html.
Friedmann, Scientific American, pp. 96-101, Jun. 1997.
Verma et al., Nature, Vol. 389, pp. 239-242, Sep. 1997.
Rubanyi, Molecular Aspects of Medicine, vol. 22, pp. 113-142, 2001.
Kappell, Current Opinions in Biotechnology, vol. 3, pp. 548-553, 1992.
M. Aritomi et al., “Crystal Structure of Rat Bc1-xL”, J. Biol. Chem., vol. 272, No. 44, pp. 27886-27892, 1997.
W. Fang et al., “Cloning and Molecular Characterization of Mouse bc1-x in B and T Lymphocytes”, J. Immunol., vol. 153, No. 10, pp. 4388-4394, 1994.
D.A.M. Grillot et al., “Genomic Organization, Promoter Region Analysis, and Chromosome Localization of the Mouse bc1-x Gene”, J. Immunol., vol. 158, No. 10, pp. 4750-4757, 1994.
N. Shirakawa et al., “An Additional Form of Rat Bc1-x, Bc1-x β, Generated by an Unspliced RNA, Promotes Apoptosis in Promyeloid Cells”, J. Biol. Chem., vol. 271, No. 22, pp. 13258-13265, 1996.
M. Gonzalez-Garcia et al., “bc1-x is the Major bc1-x mRNA form Expressed During Murine Development and its Product Localizes to Mitochondria”, vol. 120, Development, No. 10, pp. 3033-3042, 1994.
J. L. Tilly et al., “Expression of Members of the Bc1-2 Gene Family in the Immature Rat Ovary: Equine Chorionic Gonatropin-Mediated Inhibition of Granulosa Cell Apoptosis is Associated with Decreased Bax and Constitutive Bc1-2 and Bc1-xlongMessenger Ribonucleic Acid Levels”, Endocrinology, vol. 136, No. 1, pp. 232-241, 1995.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Genetically engineered cDNA of rat bcl-x gene and an... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Genetically engineered cDNA of rat bcl-x gene and an..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Genetically engineered cDNA of rat bcl-x gene and an... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4127997

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.