Genetically biotinylated recombinant antibody in...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

Reexamination Certificate

active

10807194

ABSTRACT:
A genetically biotinylated single chain fragment variable (scFv) antibody against Venezuelan equine encephalitis virus (VEE) being applied in a system consisting of an immunofiltration-enzyme assay (IFA) with a light addressable potentiometric sensor (LAPS) for the rapid identification of VEE is disclosed. The IFA entails formation of an immunocomplex sandwich consisting of VEE, biotinylated antibody, fluoresceinated antibody and streptavidin, capturing the sandwich by filtration on biotinylated membrane, and detecting the sandwich by anti-fluorescein urease conjugate. The concentration ratio of biotinylated to fluoresceinated antibodies is investigated and optimized. The IFA/LAPS assay sensitivity was approximately equal to that of a conventional enzyme-linked immunosorbant assay utilizing polystyrene plates and a chromogenic substrate, however, less time and effort were required for performance of the IFA/LAPS assay.

REFERENCES:
Hu et al., “Genetic Engineering of Streptavidin-Binding Peptide Tagged Single-Chain Variable Fragment Antibody to Venezuelan Equine Encephalitis Virus,” Hybridoma and Hybridomics, vol. 21, No. 6, pp. 415-420, Mary Ann Liebert, Inc. (2002).
Lee et al., “Rapid Immunofiltration Assay of Newcastle Disease Virus Using a Silicon Sensor,” Journal of Immunological Methods, vol. 166, pp. 123-131, Elsevier Science Publishers, B.V. (1993).
Luo et al., “Expression of a Fusion Protein of scFv—Biotin Mimetic Peptide for Immunoassay,” Journal of Biotechnology, vol. 65, pp. 225-228, Elsevier Science B.V. (1998).
Emanuel et al., “Recombinant Antibodies: A New Reagent for Biological Agent Detection,” Biosensors & Bioelectronics, vol. 14, pp. 751-759, Elsevier Science S.A. (2000).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Genetically biotinylated recombinant antibody in... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Genetically biotinylated recombinant antibody in..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Genetically biotinylated recombinant antibody in... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3850428

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.