Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Reexamination Certificate
2007-12-18
2007-12-18
Campell, Bruce R. (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
Reexamination Certificate
active
10807194
ABSTRACT:
A genetically biotinylated single chain fragment variable (scFv) antibody against Venezuelan equine encephalitis virus (VEE) being applied in a system consisting of an immunofiltration-enzyme assay (IFA) with a light addressable potentiometric sensor (LAPS) for the rapid identification of VEE is disclosed. The IFA entails formation of an immunocomplex sandwich consisting of VEE, biotinylated antibody, fluoresceinated antibody and streptavidin, capturing the sandwich by filtration on biotinylated membrane, and detecting the sandwich by anti-fluorescein urease conjugate. The concentration ratio of biotinylated to fluoresceinated antibodies is investigated and optimized. The IFA/LAPS assay sensitivity was approximately equal to that of a conventional enzyme-linked immunosorbant assay utilizing polystyrene plates and a chromogenic substrate, however, less time and effort were required for performance of the IFA/LAPS assay.
REFERENCES:
Hu et al., “Genetic Engineering of Streptavidin-Binding Peptide Tagged Single-Chain Variable Fragment Antibody to Venezuelan Equine Encephalitis Virus,” Hybridoma and Hybridomics, vol. 21, No. 6, pp. 415-420, Mary Ann Liebert, Inc. (2002).
Lee et al., “Rapid Immunofiltration Assay of Newcastle Disease Virus Using a Silicon Sensor,” Journal of Immunological Methods, vol. 166, pp. 123-131, Elsevier Science Publishers, B.V. (1993).
Luo et al., “Expression of a Fusion Protein of scFv—Biotin Mimetic Peptide for Immunoassay,” Journal of Biotechnology, vol. 65, pp. 225-228, Elsevier Science B.V. (1998).
Emanuel et al., “Recombinant Antibodies: A New Reagent for Biological Agent Detection,” Biosensors & Bioelectronics, vol. 14, pp. 751-759, Elsevier Science S.A. (2000).
Alvi Azhar Z.
Fulton R. Elaine
Hu Weigang
Nagata Leslie P.
Campell Bruce R.
Her Majesty the Queen in right of Canada as represented by the
Rader & Fishman & Grauer, PLLC
Salvoza M. Franco
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