Genetic transformation of zygotes

Details Genetic transformation of zygotes Genetic transformation of zygotes

1986-08-18

1989-10-10

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4351721, 4353171, 435320, 4352402, 935 53, 935 70, 800 1, C12N 1500, C12N 100, C12N 500

Patent

active

048731919

ABSTRACT:
Genetic transformation of a zygote and the embryo and mature organism which result therefrom is obtained by placing or inserting exogenous genetic material into the nucleus of the zygote or into any genetic material which ultimately forms at least a part of the nucleus of the zygote. It is preferred that the exogenous genetic material be added to a pronuclei of the zygote and is particularly preferred that it be added to the male pronucleus of the zygote. Thereafter, the zygote is allowed to undergo differentiation and development into the organism. The genotype of the zygote and the organism which results therefrom will include the genotype of the exogenous genetic material and the exogenous genetic material will be phenotypically expressed.
The invention can be utilized in a variety of ways including, for example, animal and plant breeding to modify or create new species, it can be used in epigenetics and in the understanding and treatment of genetic diseases.

REFERENCES:
Gordon et al, PNSA USA, vol. 77, pp. 7380-7384, Dec. 1980.
Wigler et al, Cell, vol. 16, pp. 777-785, Apr. 1979.
Jahner et al, Nature, vol. 287, pp. 456-458 (Oct. 1980).
Jaenish et al, PNAS, vol. 71, pp. 1250-1254 (Apr. 1974).
Lacy et al, Cell, vol. 18, pp. 1273-1283 (Dec. 1979).
Lacy et al, Cell, vol. 21, pp. 545-553 (Sep. 1980).
Hardison et al, Cell, vol. 18, pp. 1285-1297 (Dec. 1979).
Wagner et al, PNSA USA, vol. 78, pp. 6376-6380 (Oct. 1981).
Brinster, J. Exp. Med., vol. 140, pp. 1049-1056 (1974).
Mintz et al, PNAS USA, vol. 72, pp. 3585-3589 (1975).
Pafaioannou et al, Nature, vol. 258, pp. 69-73 (1975).
Pellicer et al, PNSA USA, vol. 77, pp. 2098-2102 (1980).
Watanabe et al, PNSA USA, vol. 75, pp. 5113-5117 (1978).
Illmensee et al, PNSA USA, vol. 75, pp. 1914-1981 (1978).
Jaenisch, PNAS USA, vol. 73, pp. 1260-1264 (1976).
Mulligan et al, Nature, vol. 277, pp. 108-114, Jan. 11, 1979.
Goeddel et al, Nature, vol. 281, pp. 544-548, Oct. 18, 1979.
Cline et al, Nature, vol. 284, pp. 422-425, Apr. 3, 1980.
Wigler et al, Cell, vol. 11, pp. 223-232 (1977).
Wigler et al, Cell, vol. 14, pp. 725-731 (1978).
Wigler et al, PNSA USA, vol. 76, pp. 1373-1376 (1979).
Capecchi et al, Cell, vol. 22, pp. 479-488 (1980).
Gordon, J. Exptl. Zoology, 228: 313-324 (1983).
Brinster, Cell, 27: 233-31 (Nov. 1981).
Harbers et al., Cell, 27: 233-231 (Nov. 1981).
Constantini and Lacy, Nature, 294: 92-14 (Nov. 1981).

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