Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Involving particle-mediated transfecion
Patent
1998-05-01
2000-02-01
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Involving particle-mediated transfecion
800300, 800323, 435430, 435470, C12N 1582, C12N 1531, C12N 1554, C12N 1565
Patent
active
060205385
ABSTRACT:
A method is disclosed for making genetically engineered orchid species using accelerated particle transformation. Meristem-tip tissue of orchid is cultured into a protocorm-like body (PLB). PLB is meristematic tissue and can be induced to regenerate into whole plant. In order to achieve efficient transformation it is necessary that relatively large numbers of meristematic cells are bombarded with the DNA-coated particles. After a particle-mediated transformation event, it is also important to stimulate continued meristemoid development before and during antibiotic selection and to reduce toxic effect of oxidized products of phenolics exuded after the wounding. It has been discovered that by using the techniques described in the present invention the meristematic tissue development, before and after the bombardment, can be stimulated while the toxic effect of oxidized phenolics can be reduced, resulting in successful transformation. The bombarded PLBs are multiplied and selected for the presence of gene products coded by the introduced genes. Shoot regeneration is then induced from the PLBs and transgenic seedlings are produced. Preferred plants for carrying out the present invention are the members of the genus Cymbidium.
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Han Kyung-Hwan
Yang Jaemo
Fox David T.
Korea Kumho Petrochemical Co. Ltd.
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