Genetic transformation of maize cells by electroporation of cell

Details Genetic transformation of maize cells by electroporation of cell Genetic transformation of maize cells by electroporation of cell

1990-12-28

1995-01-24

Benzion, Gary

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

43524047, 43524049, 43524050, 43524046, 800205, 935 67, C12N 1500, C12N 1505

Patent

active

053842537

ABSTRACT:
The present invention provides a method to increase the susceptibility of cultured Zea mays cells to stable transformation with recombinant DNA via electroporation, by pretreating the Zea mays cells with certain pectin-degrading enzymes, so that the cells retain their ability to regenerate fertile, transgenic Zea mays plants containing the DNA which is also heritable.

REFERENCES:
C. Rhodes, "Plant Regeneration from Protoplasts Isolated from Embryogenic Maize Cell Cultures", Bio/Technology, 6:56-60 (1988).
S. Morocz et al., "Two Approaches to Rendering Zea mays L. Applicable to Tissue Culture Manipulations", Abstract VIIth International Congress on Plant Tissue and Cell Culture, Amsterdam A1-102, Abstract No. 209, p. 190 (1990).
I. Potrykus, Biotechnology, 535, 2 (1990).
R. A. Dekeyser et al., Plant Cell, 2, 591 (1990).
G. Donn et al., Abstracts VIIth International Congress on Plant Tissue and Cell Culture, Amsterdam A2-38 (1990).
I. Potrykus, Physiologia Plantarum, 79, 125 (1990).
K. Lindsey et al., Physiologia Plantarum, 79 168 (1990).
K. Lindsey et al., Plant Cell Reports, 8, 71 (1989).
J. Lee et al., Korean J. Genetics, 11, 65 (1989).
Y. Huang et al., Plant Cell, Tissue and Organ Culture, 18, 281 (1989).
K. Shimamoto et al., Nature, 338, 274 (1989).
H. Jones et al., Plant Mol. Biol., 13, 503 (1989).
M. Horn et al., Plant Cell Reports 7, 469 (1988).
K. Schimamoto et al., International Soc'y for Plant Molec. Biol., 2nd International Congress, Jerusalem (1988).
C. Rhodes et al., Science, 240, 204 (1988).
K. Weising et al., Annu. Rev. Genet., 22, 421 (1988).
H. Yang et al., Plant Cell Reports, 7, 421 (1988).
K. Shigekawa et al., BioTechniques, 6, 742, (1988).
K. Lindsey et al., Plant Molecular Biol., 10, 43 (1987).
E. Cocking et al., Science, 236, 1259 (1987).
H. Jones et al., Oxford Surveys of Plant Molecular & Cell Biol., 4, 347 (1987).
H. Morikawa et al., Gene, 121 (1986).
M. Fromm et al., Nature, 319, 791 (1986).
M. Fromm et al., PNAS USA, 82, 5824 (1985).
H. Potter et al., PNAS USA, 81, 7161 (1984).

Affiliated with

Also associated with

Rating

Genetic transformation of maize cells by electroporation of cell does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Genetic transformation of maize cells by electroporation of cell, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Genetic transformation of maize cells by electroporation of cell will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1468245