Genetic sequences encoding dominant-negative chalcone...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S023100, C536S023200, C800S298000

Reexamination Certificate

active

07049427

ABSTRACT:
The invention includes modifiedMazuschalcone synthase (CHS) nucleic acids, which encode a modified chalcone synthase that has alanine instead of cysteine at the 165thamino acid ofMazusCHS and either glycine or lysine instead of methionine at the 138thamino acid ofMazusCHS. The property of the encoded modifiedMazusCHS is characterized by its dominant-negative inhibition of CHS. The invention also includes plants having at least one cell expressing the modifiedMazusCHS. Such plants are characterized by the decreased content of anthocyanins. The invention also includes vectors comprising at least a portion of the modifiedMazusCHS nucleic acids. The invention also includes methods using such vectors for producing plants having the decreased content of anthocyanins.

REFERENCES:
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GenBank Accession AB012923 Jan. 17, 2001.
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Ferrer et al, Nature Structural Biology, vol. 6, No. 8, Aug. 1999, pp. 775-784, Structure of chalcone synthase and the . . . .
Holton et al, The Plant Cell, vol. 7, Jul. 1995, pp. 1071-1083, Genetics and Biochemistry of Anthocyanin Biosynthesis.
Jorgensen et al, Plant Molecular Biology 31, 1996, pp. 957-973, Chalcone synthase cosuppression phenotypes in petunia flowers . . . .
Palauqui et al, The EMBO Journal, vol. 16, No. 15, 1997, pp. 4738-4745, Systemic acquired silencing: transgene-specific . . . .
Tanaka et al, Plant Cell Physiol. 39(11), 1998, pp. 1119-1126, Metabolic Engineering to Modify Flower Color.
Vaucheret et al, Journal of Cell Science 114, 2001, pp. 3083-3091, Post-transcriptional gene silencing in plants.
Voinnet al, NATURE, vol. 389, Oct. 9, 1997, p. 553, Systemic signalling in gene silencing.
Voinnet et al, CELL, vol. 95, Oct. 16, 1998, pp. 177-187, Systemic Spread of Sequence-Specific Transgene RNA Degradation in . . . .

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