Genetic sequence assay using DNA triple strand formation

Details Genetic sequence assay using DNA triple strand formation Genetic sequence assay using DNA triple strand formation

1993-12-22

1999-01-19

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 243, 935 77, 935 78, C12Q 168, C07H 2104, C07H 2102

Patent

active

058612440

ABSTRACT:
A method of assaying genetic sequences comprises introducing double stranded DNA to be assayed into an aqueous buffer medium containing complexes of an anchor DNA strand anchored to a support matrix and hybridized with a reporter DNA strand having a detectable label. A portion of the anchor strand or the reporter strand consists of a selected sequence of bases capable of forming a triple helix or triple strand structure with a portion of particular double stranded DNA. The reporter strand is displaced from the complex upon formation of a triple helix or triple strand structure, and displaced reporter strands are detected to determine the presence of the particular double stranded DNA. Gene probes, anchor/reporter hybrids, selected gene sequences and kits, useful in the assay method, are provided.

REFERENCES:
patent: 4436826 (1984-03-01), Wang
patent: 4454233 (1984-06-01), Wang
patent: 4766062 (1988-08-01), Diamond et al.
patent: 5422251 (1995-06-01), Fresco
patent: 5460941 (1995-10-01), Camerini-Otero et al.

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