Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Cell membrane or cell surface is target
Patent
1996-10-11
1999-06-29
Degen, Nancy
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Cell membrane or cell surface is target
435419, 372 5, 372 22, 372 23, 372 25, 372 38, C12N 1300, C12N 504, H01S 330, H01S 310
Patent
active
059167882
ABSTRACT:
A genetic recombination laser apparatus includes an optical system having a semiconductor laser medium for generating a semiconductor laser light, an Nd:YAG crystal, an LBO and .beta.-BBOs for reducing the wavelength of the laser light, an acoustic optical device and a lens for converging the laser light with a reduced wavelength;. A stage for positioning the focus of the laser light converged by the lens at any arbitrary location on a specimen is provided. The energy of the laser light converged on the specimen is set at a value greater than the bonding energy of the substance composing the wall of the somatic cell. Preferably, the semiconductor laser light is converted into the fifth harmonic of an Nd:YAG laser light having a wavelength of 213 nm and a second harmonic with a wavelength of 532 nm generated in the conversion process is used as for positioning the focus of the laser light converged by the lens on the specimen. The laser apparatus is capable of boring a hole through the wall of a somatic cell of the higher plant for injecting foreign genetic material into the interior of the somatic cell to undergo genetic transformation.
REFERENCES:
patent: 5114630 (1992-05-01), Lin
patent: 5272072 (1993-12-01), Kaneko et al.
patent: 5570384 (1996-10-01), Nishida et al.
Weber et al.. Genetic micromanipulation of plant cells and organelles with a laser microbeam. Plant Cell, Tissue and Organ Culture. vol. 12:219-222, Mar. 11, 1988.
Weigand et al. Laser-induced fusion of mammalian cells adn plant protoplasts. J. Cell Science. vol. 88:145-149, Jan. 1987.
Kubota Shigeo
Liu Ling Yi
Wiechmann Werner
Degen Nancy
Sandals William
Sony Corporation
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