Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1983-06-27
1986-02-18
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4351721, 435236, 435237, 435948, 424 89, 424 86, 935 65, 935111, 935 95, C12N 1500, C12N 704, C12N 708, C12R 191
Patent
active
045713855
ABSTRACT:
This invention relates to processes which are used to produce, isolate, and characterize human rotavirus/animal rotavirus reassortants and to produce live attenuated vaccines and vaccine precursors. In the present strategy there is involved the new use of either (1) high titer hyperimmune antisera or (2) monoclonal antisera to select reassortants with the desired human phenotype. A point of novelty is the finding that antiserum or monoclonal antisera alone, so long as it possesses high titer neutralizing activity against only the 34-38Kd glycoprotein or of the animal parent, is sufficient to use for selection of reassortant rotaviruses with human phenotype. Also, the novel products are live attenuated vaccine precursors and vaccines.
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Chanock Robert M.
Greenberg Harry B.
Kalica Anthony R.
Kapikian Albert Z.
Midthun Karen
Roberts, Jr. John S.
Tarcza John Edward
The United States of America as represented by the Department of
Wiseman Thomas G.
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