Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1984-04-18
1988-01-19
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4351723, 536 27, 935 4, 935 9, 935 13, 935 78, C12Q 168, C12N 1500
Patent
active
047204540
ABSTRACT:
A cDNA clone is isolated encoding a bovine adrenal cytochrome P-450 specific for steroid 21-hydroxylation (P-450.sub.C21). This plasmid pC21a contains an insert of 520 base pairs. It hybridizes with mRNA encoding P-450.sub.C21. The peptide encoded by the insert is highly homologous to two peptides isolated from porcine P-450.sub.C21 and shows limited homology to the P-450 induced by phenobarbital in rat liver. This probe and its human equivalents can be used to screen in utero i.e. pre-natal as well as post-natal patients for adrenal hyperplasia since it reacts well with human DNA as shown. These probes can also be useful for an industrial fermentation process to produce cortisone in quantity.
REFERENCES:
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Yuan et al, PNAS, vol. 80, 1169-1173, 1983.
Marville et al, J. of Biol. Chem., vol. 258, No. 6, 3901-3906, 1983.
Fujii-Kuriyama, PNAS, vol. 79, 2793-2797, 1982.
White et al, PNAS, vol. 81, pp. 1986-1990, Apr. 1984.
White et al, PNAS, vol. 83, pp. 5111-5115, Jul. 1986.
Dupont Bo
New Maria I.
White Perrin C.
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