Genetic markers and methods for the detection of escherichia col

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 243, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2104

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057472572

ABSTRACT:
A method, diagnostic sequences and primers are provided that are useful in the identification of the Escherichia coli 0157:H7 serotype. The method first involves the identification of a RAPD-amplified DNA fragment common to 0157:H7 Escherichia coli, the identification of the most conserved regions of that fragment, and the preparation of specific primers useful for detecting the presence of a marker within the fragment whereby that set of primers is then useful in the identification of all 0157:H7 Escherichia coli.

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