Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1989-03-15
1991-08-20
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 91, 935 78, C12Q 168, C12N 120, C12P 1934
Patent
active
050413716
ABSTRACT:
An assay for a genetic marker associated with increased milk production is disclosed. Also disclosed are kits for use in connection with the assay and breeding methods that use the assay. The assay centers on finding a genetic marker in a bovine cell (e.g. in the DNA of the cell). The presence of the marker is confirmed by exposing a gene sequence from the cell to a restriction enzyme so as to yield gene fragments of varying lengths. During a separation step there is a separation of some of the fragments from others (such as by using electrophoresis), and there is then a hybridization of a plurality of probes that contain a portion of bovine prolactin sequence to the separated fragments. The probe is radiolabelled. Then, there is a comparison of the results of the hybridization with the hybridization results for a gene sequence known to either have the marker or not have the marker. The assay appears to be of greatest utility in connection with the Carlin-M Ivanhoe Bell Holstein family.
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Ax Roy L.
Cowan Charles M.
Dentine Margaret R.
Schuler Linda A.
Escallon Miguel
Wax Robert A.
Wisconsin Alumni Research Foundation
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