Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2006-05-02
2006-05-02
Nelson, Amy J. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C800S278000, C800S298000
Reexamination Certificate
active
07038113
ABSTRACT:
Soybean andMedicago truncatulaCYP93C genes have been isolated which encode a cytochrome P450 that can catalyze the aryl migration of a flavanone to yield an isoflavanone intermediate or an isoflavone. Plants can now be genetically engineered to produce isoflavones that provide potential human health benefits and increase disease resistance in plants. Isoflavones can now be produced in transgenic plants species in which isoflavones do not naturally occur, i.e., in species other than legumes. Alternatively, introducing infection-inducible isoflavonoid biosynthesis into non-legumes qualitatively complements these plants phytoalexin defenses against microbial pathogens, whereas over-expression of the isoflavonoid pathway in legumes quantitatively increases this defense response. Finally, modifying the extend of production of isoflavonoids in legume roots positively impacts nodulation efficiency and therefore plant yield.
REFERENCES:
patent: WO9615239 (1996-05-01), None
patent: WO9919493 (1999-04-01), None
Siminszky B. et al. PNAS, Feb. 1999, vol. 96, pp. 1750-1755.
Liu C. et al. The Plant Journal, 2003; vol. 36 pp. 471-484.
Liu C. et al. PNAS, 2002, vol. 99, No. 22; pp. 14578-14583.
Adlercreutz, et al., 1991, “Urinary excretion of lignans and isoflavonoid phytoestrogens in Japanese men and women consuming a traditional Japanese diet,”Am J Clin Nutr54:1093-1100.
Akashi, et al, 1998, “Identification of a cytochrome P450 cDNA encoding (2S)-flavanone 2-hydroxylase of licorice (Glycyrrhiza echinataL.:Fabaceae) which represents licodione synthase and flavone synthase II,”FEBS Letters431: 287-290.
Akashi, T., et al., 1999, “Cloning and Functional Expression of a Cytochrome P450 cDNA Encoding 2-Hydroxyisoflavanone Synthase Involved in Biosynthesis of the Isoflavonoid Skeleton in Licorice,”Plant Physiology, 121:821-828.
Akiyama, et al., 1987, “Genistein, a specific inhibitor of tyrosine-specific protein kinases,”J Biol Chem262: 5592-559.
Angell, S. M. and D. C. Baulcombe, 1997, “Consistent gene silencing in transgenic plants expressing a replicating potato virus X RNA,”EMBO J16:3675-3684.
Arora, et al., 1998, “Antioxidant activities of isoflavones and their biological metabolites in a lipsomal system,”Arch Biochem Biophys356: 133-141.
Bhattacharyya, M. K. and E. W. B. Ward, 1987, “Biosynthesis and metabolism of glyceollin I in soybean hypocotyls following wounding or inoculation withPhytophthora megaspermaf. sp.glycinea,” Physiol and Mol Plant Pathology31: 387-405.
Bourque, J.E., 1995, “Antisense strategies for genetic manipulation in plants,”Plant Science105:125-149.
Clough, S.J. and Bent, A.F., 1998, “Floral dip: a simplified method forAgrobacterium-mediated transformation ofArabidopsis thaliana”, Plant J16:735-74.
Colliver, S.P., et al., 1997, “Differential modification of flavonoid and isoflavonoid biosynthesis with an antisense chalcone synthase construct in trasgenicLotus corniculatus,” Plant Molecular Biology, 35: 509-522.
Coward, et al., 1993, “Genistein, daidzein, and their β-glycoside conjugates: antitumor isoflavones in soybean foods from American and Asian diets,”J Agricultural and Food Chemistry41: 1961-1967.
Dakora, et al., 1993, “Common bean root exudates contain elevated levels of daidzein and coumestrol in response toRhizobiuminoculation,”Mol Plant-Microbe Interact6: 665-668.
Dixon, R. A. and N. L. Paiva, 1995, “Stress-induced phenylpropanoid metabolism,”Plant Cell7: 1085-1097.
Dixon, R., et al., 1995, “The isoflavonoid phytoalexin pathway: From enzymes to genes to transcription factors,”Physiologia Plantarum, 93:385-392.
Dixon, R.A., Blyden, E.R., Robbins, M.P., van Tunen, A.J. and Mol, J.N.M., 1988, “Comparative biochemistry of chalcone isomerases.”Phytochemistry27: 2801-2808.
Dixon, R.A., et al., 1999 “Molecular controls for isoflavonoid biosynthesis in relation to plant and human health,”Phytochemicals in Human Health Protection, Nutrition and Plant Defense, Romeo, ed., Kluwer Academic/ Plenum Publishers, New York; pp. 133-159.
Draper, et al., 1997, “Phytoestrogens reduce bone loss and bone resorption in oophorectomized rats,”J Nutr127: 1795-1799.
Graham, T.L., 1991, “Flavonoid and isoflavonoid distribution in developing soybean seeding tissues and in seed and root exudates.”Plant Physiol95: 594-603.
Graham, T.L., 1998, “Flavonoid and flavonol glycoside metabolism inArabidopsis”. Plant Physiol Biochem36: 135-144.
Hadwiger, L. A. and D. M. Webster, 1984, “Phytoelexin production in five cultivars of pea differentially resistant to three races ofPseudomonas syringaepv.pisi,” Phytopathology74: 1312-1314.
Hagmann, M. and H. Grisebach, 1984, “Enzymatic rearrangement of flavanone to isoflavone,”FEBS Letters175: 199-202.
Hakamatsuka, et al., 1991, “P-450-dependent oxidative rearrangement in isoflavone biosynthesis: reconstitution of P-450 and NADPH:P450 reductase,”Tetrahedron47: 5969-5978.
Hakamatsuka, et al., 1998, “Purification of 2-hydroxyisoflavanone dehydratase from the cell cultures ofPueraria lobata,” Phytochemistry49: 497-505.
Hashim, et al., 1990, “Reaction Mechanism of oxidative rearrangement of flavanone in isoflavone biosynthesis,”FEBS Letters271: 219-222.
Horsch, et al., 1985, “A simple and general method for transferring genes into plants,”Science227:1229-1231.
Jung, W., et al., 2000, “Identification and expession of isoflavone synthase, the key enzyme for biosynthesis of isoflavones in legumes,”Nature Biotechnology18: 206-212.
Kape, et al., 1992, “Legume root metabolites and VA-mycorrhiza development,”J Plant Physiol141: 54-60.
Kessmann, et al., 1990, “Stress responses in alfalfa (Medicago sativaL.) III. Induction of medicarpin and cytochrome P450 enzyme activities in elicitor-treated cell suspension cultures and protoplasts,”Plant Cell Reports9: 38-41.
Keung, et al., 1995, “Daidzin suppresses ethanol consumption by Syrian golden hamsters without blocking acetaldehyde metabolism,”Proc Natl Acad Sci USA92: 8990-8993.
Keung, W. M. and B. L. Vallee, 1993, “Daidzin: A potent, selective inhibitor of human mitochondrial aldehyde dehydrogenase,”Proc Natl Acad Sci USA90: 1247-1251.
Kirikae, et al., 1993, “Biosynthesis of a dibenzoylmethane, licodione, in cultured alfalfa cells induced by yeast extract,”Biosci Biotech Biochem57: 1353-1354.
Klein, et al., 1988, “Stable genetic transformation of intactNicotianacells by the particle bombardment process,”Proc Natl Acad Sci USA85:8502-8505.
Kochs, G. and H. Grisebach, 1986, “Enzymic synthesis of isoflavones,”European J Biochem155: 311-318.
Kochs, G. and H. Grisebach, 1987, “Induction and characterization of a NADPH-dependent flavone synthase from cell cultures of soybean,”Z. Naturforsch42C: 343-348.
Kosslak, et al., 1987, “Induction ofBradyrhizobium japonicumcommon nod genes by isoflavones isolated fromGlycine max,” Proc Natl Acad Sci USA84: 7428-7432.
Köster, J. and W. Barz, 1981, “UDP-Glucose: isoflavone 7-O-glucosyltransferase from roots of chick pea (Cicer arietinumL.).”Arch Biochem Biophys212: 98-104.
Lee, et al., 1991, “Dietary effects on breast-cancer risk in Singapore,”Lancet337: 1197-1200.
Long, et al., 1985, “Further studies on the relationship between glyceollin accumulation and the resistance of soybean leaves toPseudomonas syringaepv.glycinea,” Phytopathology75: 235-239.
Martens, S. and G. Forkmann, “Cloning and expression of flavone synthase II from Gerbera hybrids,”Plant J20: 616-618.
Martin, et al., 1996, “Interactions between phytoestrogens and human sex steroid binding protein,”Life Sciences58: 429-436.
Nelson, et al. 1993. “The P450 superf
Dixon Richard A.
Steele Christopher L.
Fulbright & Jaworski L.L.P.
Kallis Russell
Nelson Amy J.
The Samuel Roberts Noble Foundation, Inc.
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