Genetic fingerprinting of yeasts

Details Genetic fingerprinting of yeasts Genetic fingerprinting of yeasts

1992-03-20

1995-04-18

Parr, Margaret

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 536 243, 536 2432, 536 2433, C12P 1934, C12Q 168, C07H 2104

Patent

active

054078148

ABSTRACT:
The present invention relates to the identification of yeast strains by way of application of the polymerase chain reaction to amplify nucleic acid sequences characteristic of their TY transposon long terminal repeats. Polymerase chain reaction product is analysed, conveniently by agarose gel electrophoresis, and its nature related to the presence of a particular yeast strain or strain type.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
Nelson et al., PNAS 86, 6686-6690 (1989).
Kingsman et al., J. Mol. Biol. 145, 619-632 (1981).
Chisholm et al., Proc. Natl. Acad. Sci USA 81, 2965-2969 (1984).
Boeke et al., Mol. Cell. Biol. 8(4), 1432-1442 (1988).
Hanson et al., Mol. Cell. Biol. 8(12), 5245-5256 (1988).
Lochmuller, et al., "A hot-spot for transposition of various Ty elements on chromosome V in Saccharomyces cerevisiae", Current Genetics, (1989) 16:247-252.

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