Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2007-08-14
2007-08-14
Kubelik, Anne (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C800S278000, C536S023600, C435S320100, C435S468000, C424S093200
Reexamination Certificate
active
11065977
ABSTRACT:
The invention is isolated nucleic acid molecules encoding Na+/H+exchanger polypeptides with at least 95% homology to that ofArabidopsis thalianafor extrusion of monovalent cations from the cytosol of cells to provide the cell with increased salt tolerance. Crop species transformed with the nucleic acid molecule are capable of surviving in soil with high salt levels that would normally inhibit growth of the crop species.
REFERENCES:
patent: 4616100 (1986-10-01), McHughen et al.
patent: 5272085 (1993-12-01), Young et al.
patent: 5346815 (1994-09-01), Krulwich et al.
patent: 5441875 (1995-08-01), Hediger
patent: 5563246 (1996-10-01), Krulwich et al.
patent: 5563324 (1996-10-01), Tarczynski et al.
patent: 5639950 (1997-06-01), Verma et al.
patent: 5689039 (1997-11-01), Becker et al.
patent: 5750848 (1998-05-01), Kruger et al.
patent: 5780709 (1998-07-01), Adams et al.
patent: 5859337 (1999-01-01), Gasser et al.
patent: 2002/0083487 (2002-06-01), Fukuda et al.
patent: 2003/0046729 (2003-03-01), Blumwald et al.
patent: 2005/0028235 (2005-02-01), Zhang et al.
patent: 2005/0144666 (2005-06-01), Blumwald et al.
patent: 2005/0155105 (2005-07-01), Blumwald et al.
patent: 2005/0204430 (2005-09-01), Blumwald et al.
patent: 1143002 (2001-10-01), None
patent: WO 91/06651 (1991-05-01), None
patent: WO 96/39020 (1996-12-01), None
patent: WO 97/13843 (1997-04-01), None
patent: WO 99/47679 (1999-09-01), None
patent: WO 00/37644 (2000-06-01), None
Yokoi et al, 2002, Plant J. 30:529-539.
Guo et al, 2004, Proc. Natl. Acad. Sci. USA 101: 9205-9210.
Nakamura et al, 1998, DNA Res. 5:297-308.
U.S. Appl. No. 10/617,624, filed Jul. 10, 2003, Blumwald.
U.S. Appl. No. 10/620,061, filed Jul. 14, 2003, Blumwald et al.
U.S. Appl. No. 10/944,174, filed Sep. 16, 2004, Fukuda et al.
APSE et al. (2002) “Engineering salt tolerance in plants” Current Opinion in Biotechnology 13: pp. 146-150.
Apse et al. (1999) “Salt tolerance conferred by overexpression of a vauolar Na+/H+ antiport in Arabidopsis” Science 285 (5431): pp. 1256-1258.
Apse et al. (1998) “Cloning and Characterization of Plant Sodium/Proton Antiports” 11 International Workshop on Plant Membrane Biology, Aug. 1998, Cambridge, U.K. (Abstract).
Apse et al. (1998) “Identification of two putative sodium/proton antiports in Arabidoposis” Plant Membrane Biology Workshop Aug. 1998, Cambridge, U.K. (Poster).
Barkla et al. (1995) “Tonoplast Na+/H+ antiport activity and its energization by the vacoular H+ -ATPase in the halophytic plant Mesembryanthemum crystallinum L” Plant Physiol. 109: pp. 549-556.
Barkla et al. (1994) “The plant vacuolar Na+/H+ antiport” Symp. Soc. Exp. Biol. 48: pp. 141-153.
Blumwald (2000) “Sodium transport and salt tolerance in plants” Current Opinion in Cell Biology 12: pp. 431-434.
Blumwald et al. (Dec. 1998) “Cloning of plant sodium/proton antiports in Arabidopsis” Eastern Regional Meeting of the Canadian Society of Plant Physiologists, Toronto.
Blumwald et al. (Jun. 1998) “Cloning and characterization of a plant sodium/proton antiport” Annual Meeting of the American Society of Plant Physiologists, Madison, USA.
Blumwald et al. (Aug. 1998) “Cloning and characterization of a plant sodium/proton antiports” 11 International Workshop on Plant Membrane Biology, Aug. 1998, Cambridge, U.K.
Blumwald et al. (Aug. 1998) “Cloning and characterization of a plant sodium/proton antiports” Gordon Conference on Drought and Salinity Stress in Plants, Oxford, UK.
Bohnert et al. (1996) “Strategies for engineering water-stress tolerance in plants” Trends in Biotechnology 14(3): pp. 89-97.
Borgese et al. (1992) “Cloning and expression of a cAMP-activated Na+/H+ exchanger: evidence that the cytoplasmic domain mediates hormonal regulation” PNAS USA 89: pp. 6765-6769.
Bork (2000) “Powers and Pitfalls in Sequence Analysis: the 70% Hurdle” Genome Research, vol. 10: pp. 398-400.
Bowie et al. (1990) “Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions” Science, vol. 247, pp. 1306-1310.
Brant et al. (1997) Human Na+/H+ exchanger isoform NHE3 composite cDNA: GenBank Accession No. T51330.
Broun et al. (1998) “Catalystic Plasticity of Fatty Acid Modification Enyzmes Underlying Chemical Diversity of Plant Lipids” Science, vol. 282: pp. 1315-1317.
Counillon et al. (May 1993) “A Point Mutation of the Na+/H+ Exchanger Gene (NHE1) and Amplification of the Mutated Allele Confer Amiloride Resistance Upon Chronic Acidosis” Proc. Natl. Acad. Sci. USA 90(10): pp. 4508-4512.
Covitz et al. (Nov. 1997) Expressed sequence tags from a root hair-enriched Medicago truncatula cDNA library: GenBank Accession No. AA660573.
Dante et al. (1997) “AC 004655”:Arabidopsis thalianaBAC TM021B04: EMBL Database Accession No. AC 004655.
Darley et al. (1998) “ANA1 a Na+/H+ Antiporter From Arabidopsis?” 11th International Workshop on Plant Membrane Biology, Aug. 1998, Cambridge, U.K.
Dietrich et al. (1997) Sequence of s. cerevisiae lambda 3641 and cosmids 9461, 9831, and 9410: GenBank Accession No. 927695.
Fukuda et al. (Aug. 1999) “AB021878”Oryza sativa(Japonica cultivar-group) OsNHX1 mRNA: EMBL Database Accession No. AB021878.
Fukuda et al. (1999) “Molecular Cloning and Expression of the Na+/H+ Exchanger Gene inOryza Sativa” Biochim. Biophys. Acta. 1446 (1-2): pp. 149-155.
Fukuda et al. (1998) “Na+/H+ Antiporter in Tonoplast Vesicles from Rice Roots” Plant Cell Physiol. 39: pp. 196-201.
Fukuda et al. (Mar. 2001) “The Functional analysis of the rice Na+/H+ antiporter gene” Plant Cell Physiol. 42 (Supp.): p. s210.
Gaxiola et al. (1996) “TheArabidopsis thalianaproton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast” PNAS USA 96 (4): pp. 1480-1485.
Gordon-Kamm et al. (1990) “Transformation of Maize Cells and Regneration of Fertile Transgenic Plants” Plant Cell 2: 603-618.
Hahnnenberger et al. (1996) “Functional expression of the Schizosaccharomyces pombe Na+/H+ antiporter gene, sod2, inSaccharomyces cerevisiae” PNAS USA 93: pp. 5031-5036.
Hiei et al. (1994) “Efficient Transformation of rice mediated by Agrobacterium and sequence analysis of the boundary of the T-DNA” Plant J. 6: pp. 271-282.
Hill et al. (1998) “Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosporylase fromEscherichia coli” Biochem. Biophys. Res. Comm. 244: pp. 573-577.
Ichida et al. (1996) “Increased Resistance to Extracellular Cation Block by Mutation of the Pore Domain of the Arabidopsis Inward-rectifying K+ Channel KAT1” J. Membrane Biol. 151: pp. 53-62.
Jacoby (Aug. 23, 1999) “Botanists design plants with a taste for salt” Chemical Engineering News: p. 9.
Kadyrzhanova et al. (1995) Sequences for STS primer sets: GenBank Accession No. L44032.
Kaufman (Jul. 31, 2001) “A New Strain of Tomatoes, And Don't Hold the Salt” Washington Post: p. A03.
Kinclova et al. (2001) “Functional study of theSaccharomyces cerevisiaeNha1p C-terminus” Mol. Microbiol. 40 (3): pp. 656-668.
Lazar et al. (1988) “Transforming Growth Factor a: Mutation of Aspartic Acid 47 and Leucine 48 Results in Different Biological Activities” Molecular and Cellular Biology 8: pp. 1247-1252.
Liu et al. (2000) “Partial Deletion of a Loop Region in the High Affinity K+ Transporter HKT1 Chages Ionic Permeability Leading to Increased Salt Tolerance” J. Biol. Chem. 275 (36): pp. 27924-27932.
Nass and Rao (Aug. 1998) “Novel Localization of a Na+/H+ Exchanger in a late Endosomal Compartment of Yeast” J. Biol. Chem. 273 (33): pp. 21054-21060.
Nass et al. (Oct. 1997) “Intracellular Sequestration of Sodium by a Novel Na+/H+ Exchanger in Yeast Is Enhanced by Mutations in the Plasma Membrane H+—ATPase” J. B
Aharon Gilad
Apse Maris
Blumwald Eduardo
Snedden Wayne
LandOfFree
Genetic engineering salt tolerance in crop plants does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Genetic engineering salt tolerance in crop plants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Genetic engineering salt tolerance in crop plants will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3884051