Prosthesis (i.e. – artificial body members) – parts thereof – or ai – Implantable prosthesis
Patent
1997-09-04
2000-06-20
Yu, Mickey
Prosthesis (i.e., artificial body members), parts thereof, or ai
Implantable prosthesis
4351721, 4351723, 424422, 424423, 623 16, A61F 202, A61F 228
Patent
active
06077987&
ABSTRACT:
A method for enhancing and/or increasing the efficiency of repair of tissues, primarily bone or cartilage, using genetically engineered cells has been developed. In the preferred embodiment, mesenchymal stem cells are isolated from periosteum tissue, and transfected with the gene encoding a growth factor for the particular cell type to be repaired. For example, for repair of bone, a gene (or genes) encoding bone morphogenic protein is transfected into periosteal cells. The transfected periosteal cells then express the bone morphogenic protein in culture to promote bone repair as a function of the expressed bone morphogenic protein. Cells can be transfected using any appropriate means, including viral vectors, as shown by the example, chemical transfectants, or physico-mechanical methods such as electroporation and direct diffusion of DNA. Genes can encode any useful protein, for example, a specific growth factor, morphogenesis factor, a structural protein, or a cytokine which enhances the temporal sequence of wound repair, alters the rate of proliferation, increases the metabolic synthesis of extracellular matrix proteins, or directs phenotypic expression in endogenous cell populations. Representative genes encoding proteins include bone growth factor genes, cartilage growth factor genes, nerve growth factor genes, and general growth factors important in wound healing, such as platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF-1), epidermal growth factor (EGF), basic fibroblast growth factor (FGF), endothelial derived growth supplement.
REFERENCES:
patent: 1995970 (1935-03-01), Dorough
patent: 2676945 (1954-04-01), Higgins
patent: 2683136 (1954-07-01), Higgins
patent: 2703316 (1955-03-01), Schneider
patent: 2758987 (1956-08-01), Salzberg
patent: 2951828 (1960-09-01), Zeile et al.
patent: 3371069 (1968-02-01), Takashi et al.
patent: 3531561 (1970-09-01), Trehu
patent: 4846835 (1989-07-01), Grande
patent: 5041138 (1991-08-01), Vacanti et al.
patent: 5226914 (1993-07-01), Caplan et al.
patent: 5492697 (1996-02-01), Boynan et al.
patent: 5563124 (1996-10-01), Damien et al.
patent: 5591453 (1997-01-01), Ducheyne et al.
patent: 5632745 (1997-05-01), Schwartz
patent: 5693341 (1997-12-01), Schroeder et al.
patent: 5763416 (1998-06-01), Bonadio et al.
Michael J. Yaszemski, Richard G. Payne, Wilson C. Hayes, Robert Langer and Antonios G. Mikos. Evolution of bone transplantation: molecular, cellular and tissue strategies to engineer human bone. Biomaterials 17 (1996) 175-185, Dec. 1996.
Allcock, et al., "Synthesis and Hydrolysis of Hexakis(imidazolyl)cyclotriphosphazene," J. Am. Chem. Soc. 103:2250-2256 (1981).
Beresfor, et al., "Futher Characterisation of a System for the Culture of Human Bone Cells," Calcif. Tissue Int. 35(4/5):637 (Abstract only) (1983).
Cheung, et al., "Growth of osteoblasts on porous calcium phosphate ceramic: an in vitro model for biocompatability study," Biomaterials 10:63-67 (1989).
Choy, et al., "Fibroblasts Behavior in the Embryonic Chick Heart," Dev. Dyn. 198(2):97-107 (1993).
Chu, "Survey of Clinically Important Wound Closure Biomaterials," in Biocompatible Polymers, Metals and Composites, (Szycher, ed.), p. 477 (Technomic Publishing Lakewood, NJ 1983).
Cohn, et al., "Isolated Bone Cells," in Skeletal Research: An Experimental Approach (Simmons, et al., ed.), pp. 3-20 (Academic Press, NY 1979).
Elgendy, et al. "Osteoblast-like cell (MC3T3-E1) proliferation on bioerodible polymers: An approach towards the development of a bone-bioerodible polymer composite material," Biomaterials 14(4):263-269 (1993).
Felgner, et al., "LipofectAmine.TM. Reagent: A New, Higher Efficiency Polycationic Liposome Transfection Reagent," Focus/Gibco 15(3):73-78 (1993).
Frame, "Hydroxyapatite as a biomaterial for alveolar ridge augmentation," Int. J. Oral Maxillofacial Surgery 16:642-655 (1987).
Friedlaender, "Current Concepts Review: Bone Grafts," Journal of Bone and Joint Surgery 69A(5):786-790 (1987).
Galileo, et al., "Neurons and glia arise from a common progenitor in chicken optic tectum: Demonstration with two retroviruses and cell type-specific antibodies," Proc. Natl. Acad. Sci. USA 87(1):458-462 (1990).
Goldberg, et al., "Effect of Gold Sodium Thiomalate on Proliferation of Human Rheumatoid Synovial Cells and on Collagen Synthesis in Tissue Culture," Biochem. Pharmacol. 29(6):869-876 (1980).
Gundberg, et al., "Measurements of Y-carboxyglutamate and circulating osteocalcin in normal children and adults," Clin. Chem. Acta 128:1-8 (1983).
Hollinger, et al., "Biodegradable Bone Repair Materials," Clinical Orthopedics and Related Research 207:290-305 (1986).
Jarcho, "Calcium Phosphate Ceramics as Hard Tissue Prosthetics," Clinical Orthopaedics and Related Research 157:259-278 (1981).
Kulkarni, et al., "Biodegradable Poly(lactic acid) Polymers," J. Biomedical Materials Research 5:169-181 (1971).
Laurencin, et al. "Use of polyphosphazenes for skeletal tissue regeneration," J. Biom. Mater. Res. 27(7):963-973 (1993).
Miller, et al., "Improved Retroviral Vectors for Gene Transfer and Expression," BioTechniques 7(9):980-990 (1989).
Miller, et al., "Redesign of Retrovirus Packaging Cell Lines to Avoid Recombination Leading to Helper Virus Production," Mol. Cel. Biol. 6(8):2895-2902 (1986).
Nabel, et al., "Direct gene transfer with DNA-liposome complexes in melanoma: Expression, biological activity and lack of toxicity in humans," Proc. Nat. Acad. Sci. USA 90(23):11307-11311 (1993).
Neville, "Molecular Weight Determination of Protein-Dodecyl Sulfate Complexes by Gel Electrophoresis in a Discontinuous Buffer System," J. Biol. Chem. 246(20):6328-6334 (1971).
Owen, "Marrow stromal stem cells," J. Cell Sci. (Suppl.) 10:63-76 (1988).
Ozkaynak, et al., "OP-1 cDNA encodes an osteogenic protein in the TGF-.beta. family," EMBO J. 9(7):2085-2093 (1990).
Parsons, et al. "Osteoconductive Composite Grouts for Orthopedic Use," Annals N.Y. Academy of Sciences 523:190-207 (1988).
Partridge, "Muscle transfection made easy," Nature 352(6338):757-758 (1991).
Pate, et al., "Isolation and Differentiation of Mesenchymal Stem Cells from Rabbit Muscle," Proc. 49th Ann. Sess. Forum. Fundamental Surg. Problems pp. 587-589 (Oct. 10-15, 1993).
Peck, et al., "Bone Cells: Biochemical and Biological Studies after Enzymatic Isolation," Science 146:1476-1477 (1964).
Peck, et al., "Cyclic 3'5' Adenosine Monophosphate in Isolated Bone Cells: Response to Low Concentration of Parathyroid Hormone," Endocrinology 92(3):692-697 (1982).
Ritsila, et al., "Periosteal and Perichondral Grafting in Reconstructive Surgery," Clin. Orthop. Related Res. 302:259-265 (1994).
Rogers, et al., "Differentiation Factors Induce Expression of Muscle, Fat, Cartilage, and Bone in a Clone of Mouse Pluripotent Mesenchymal Stem Cells," Am. Surg. 61(3):231-236 (1995).
Tuli, et al., "N.sup.o -Benzyladenine: Inhibitor of Respiratory Kinases," Science 146:1477-1479 (1964).
Wade, et al. "Biocompatibility of eight poly(organophosphazenes)," in Organomet. Polym., pp. 283-288 (Carraher, et al., eds.) (Academic Press, New York, 1978).
Wilson, "Vehicles for gene therapy," Nature 365(6448):691-692 (1993).
Wivel, et al., "Germ-Line Gene Modification and Disease Prevention: Some Medical and Ethical Perspectives," Science 262:533-538 (1993).
Wolff, et al., "Direct Gene Transfer into Mouse Muscle in Vivo," Science 247:1465-1468 (1990).
Wolff, "Human dystrophin expression in mdx mice after intramuscular injection of DNA constructs," Nature 352(6338):815-818 (1991).
Woo, et al., "In Vivo Gene Therapy of Hemophilia B: Sustained Partial Correction in Factor IX-Deficient Dogs," Science 262:117-119 (1993).
Young, et al., "Pluripotent mesenchymal stem cells reside within avian conenctive tissue matarices," In Vitro Cell. Dev. Biol. Anim. 29A(9):723-736 (1993).
Ahrens, et al, "Expression of human bone morphogenic proteins -2 or -4 in murine mesenchymal progenitor C3H10T1/2 cells induces differentiation into distinct mesenchymal cell lineages," DNA and Cell Biology 12(10): 871-880 (1993).
Gitelman, et al, "Vgr-1
Breitbart Arnold S.
Grande Daniel S.
Mason James M.
Nguyen Tram A.
North Shore-Long Island Jewish Research Institute
Yu Mickey
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