Genetic detoxification of pertussis toxin

Details Genetic detoxification of pertussis toxin Genetic detoxification of pertussis toxin

1990-09-28

1993-09-14

Hill, Jr., Robert J.

Drug, bio-affecting and body treating compositions

Lymphokine

424 92, A61K 3910

Patent

active

052446573

ABSTRACT:
A new method is described for the preparation of a safe, immunogenic and efficacious vaccine for protection against the disease pertussis. In development of this vaccine, specific functional sites of pertussis toxin have been identified, and using this information, defined mutant holotoxins have been produced by site directed mutagenesis of the toxin gene. A number of these holotoxin analogs are detoxified, retain an immunodominant S1 epitope, are immunogenic and are protective in the standard pertussis vaccine potency test in mice.

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