Genetic detoxification of pertussis toxin

Details Genetic detoxification of pertussis toxin Genetic detoxification of pertussis toxin

1991-11-05

1994-10-25

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

4351721, 4352521, 4351723, 536 235, 536 231, 530324, 530350, C12N 100, C12N 1500, C12N 112, C07K 300

Patent

active

053588681

ABSTRACT:
A new method is described for the preparation of a safe, immunogenic and efficacious vaccine for protection against the disease pertussis. In development of this vaccine, specific functional sites of pertussis toxin have been identified, and using this information, defined mutant holotoxins have been produced by site directed mutagenesis of the toxin gene. A number of these holotoxin analogues are detoxified, retain an immunodominant S1 epitope, are immunogenic and are protective in the standard pertussis vaccine potency test in mice.

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