Genetic assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 435 74, 536 243, 536 2431, 536 2433, C12Q 168, C07H 2104

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active

059811749

ABSTRACT:
Polymorphisms at positions 100, 271, 281, 294, 408, 506 or 1432 of the cytochrome P450 enzyme bufuralol-1'-hydroxylase are indicative of the extensive metaboliser/poor metaboliser phenotypes and can be detected using known methods such as amplification of the DNA with the polymerase chain reaction, followed by digestion with a suitable restriction enzyme and analysis by gel electrophoresis. The EM/PM phenotype is relevant to calculating safe or effective drug doses for individuals.

REFERENCES:
patent: 5648482 (1997-07-01), Meyer
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Miles et al., Nucleic Acids Research, vol. 17, No. 20:8241-8255 (1989).
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Heim et al., Genomics, 14:49-58 (1992).

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