Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2006-05-23
2006-05-23
Benzion, Gary (Department: 1637)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S091100, C435S006120
Reexamination Certificate
active
07049104
ABSTRACT:
The present invention relates to a quick and simple method for genetic testing, particularly for SNP testing, using two kinds of primers which are complementary to a mutant target and a wild-type target, respectively, and different in length or migration speed in electrophoresis. These probes are allowed to hybridize with targets, fluorophore-tagged nucleotides are attached to the primers, and electrophoresis is carried out for discriminatory detection.
REFERENCES:
patent: 5595890 (1997-01-01), Newton et al.
patent: 6841055 (2005-01-01), Hayashizaki
patent: WO 01/62966 (2001-08-01), None
patent: WO 02/18659 (2001-08-01), None
Pastinen et al. Multiplex, fluorescent, solid phase minisequencing for efficient screening of DNA sequence variation. Clinical Chemistry, vol. 42, No. 9, pp. 1301-1397, Sep. 1996.
Bernat et al. Rapid identification of single nucleotide polymorphisms by fluorescence-based capillary electrophoresis. vol. 1, No. 1, pp. 72-78, Mar. 28, 2002.
Brown et al. Fluorescent, Multiplexed, automated, primer-extension asay for 3120+1G to A and I148T mutations in Cystic Fibrosis. Clinical Chemistry, vol. 47, No. 11, pp. 2053-2055, Nov. 1, 2001.
Tian et al. Rapid Detection of Deletion, Insertion, and substitution mutations via heteroduplex analysis using capillary- and microchip-based electrophoresis. Genome Research, vol. 10, pp. 1403-1413, 2000.
Matyas et al. Quantificiation of single nucleotide polymorphisms: A novel method that combines primer extension assay and capillary electrophoresis. Human Mutation, vol. 19, pp. 58-68, Jan. 2002.
Mostafa Ronaghi, Mathias Uhlen and Pal Nyren, “A Sequencing Method Based on Real-Time Pyrophosphate”, Science. vol. 281 (Jul. 17, 1998), p. 363.
Hideki Kambara. Masao Kamahori. Kazunori Okano, Guohua Zou. “DNA”. Jpn J. Elecroph. 2001:45: pp. 219-225, Abstract only.
“SNPs Analysis”. Bio Wave. vol. 17 (2001). pp. 2-5, Abstract only.
Kambara Hideki
Li Zheng Ping
Nagai Keiichi
Okano Kazunori
A. Marquez, Esq. Juan Carlos
Benzion Gary
Fisher Esq. Stanley P.
Hitachi LTD
Reed Smith LLP
LandOfFree
Genetic analysis method does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Genetic analysis method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Genetic analysis method will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3602841