Genes of strain DC413 encoding enzymes involved in...

Details Genes of strain DC413 encoding enzymes involved in... Genes of strain DC413 encoding enzymes involved in...

2007-10-30

2007-10-30

Hutson, Richard (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing a carotene nucleus

C435S320100, C435S252300, C435S254110, C435S254200, C435S257100, C536S023100

Reexamination Certificate

active

10810733

ABSTRACT:
A carotenogenic biosynthetic gene cluster has been isolated fromPanteoa stewartiistrain DC413, wherein the genetic organization of the cluster is crtE-idi-crtX-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), zeaxanthin glucosyl transferase (CrtX), lycopene cyclase (CrtY), phytoene desaturase (CrtI), phytoene synthase (CrtB), and β-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

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