Genes of carotenoid biosynthesis and metabolism and a system for

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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4352523, 43525411, 4353201, 435325, 536 232, C12N 121, C12N 902, C12N 1553, C12N 1563

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057443416

ABSTRACT:
The present invention also describes the DNA sequence for eukaryotic genes encoding .epsilon. cyclase, isopentenyl pyrophosphate isomerase and .beta.-carotene hydroxylase as well as vectors containing the same and hosts transformed with said vectors. The present invention provides methods for controlling the ratio of various carotenoids in a host and for the production of novel carotenoid pigments. The present invention also provides a method for screening for eukaryotic genes encoding carotenoid biosynthesis.

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FEBS Letters, vol. 328, No. 1-2, pp. 130-138, Aug. 1993, Francis X. Cunningham, Jr., et al., "Cloning and Functional Expression in Escherichia coli of a Cyanobacterial Gene for Lycopene Cyclase, The Enzyme That Catalyzes the Biosynthesis of .beta.-Carotene".
The Journal of Biological Chemistry, vol. 271, No. 13, Mar. 29, 1996, pp. 7774-7780, Nuria Cunillera, et al., "Arabidopsis thaliana Contains Two Differentially Expressed Farnesyl-Diphosphate Synthase Genes".
The Journal of Biological Chemistry, vol. 271, No. 40, pp. 24349-24352, Oct. 4, 1996, Zairen Sun, et al., "Cloning and Functional Analysis of the .beta.-Carotene Hydroxylase of Arabidopsis thaliana".
Annual Review of Plant Physiology and Molecular Biology, vol. 45, pp. 287-301, Glenn E. Bartley, et al., "Molecular Biology of Carotenoid Biosynthesis in Plants" (1994).

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