Genes involved in tolerance to environmental stress

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers resistance to heat or cold

Reexamination Certificate

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C800S287000

Reexamination Certificate

active

10342224

ABSTRACT:
The present invention relates to a method for obtaining polynucleic acids comprising coding sequences and/or genes involved in environmental stress resistance in plants, comprising the preparation of a cDNA library comprising coding sequences from siliques, introducing said coding sequences in yeast cells in a functional format and screening for polynucleic acids leading to an enhanced tolerance or resistance to environmental stress conditions in said transformed yeast cells. The present invention further relates to an isolated polynucleic acid obtainable by such a method as listed in Table 1 as well as recombinant polynucleic acid comprising the same. The present invention further relates to an isolated polypeptide encoded by a polynucleic acid of the invention. The present invention also relates to a method for producing a plant with enhanced tolerance or resistance to environmental stress, said method comprising introducing into a plant cell a recombinant DNA comprising a polynucleic acid as defined which when expressed in a plant cell enhances the tolerances or induces resistance to environmental stress conditions of said plant. The present invention particularly relates to plant cells, plants or harvestable parts or propagation material thereof transformed with a recombinant polynucleic acid as defined above.

REFERENCES:
Van Camp W. et al. Enhancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts. Plant Physiol. Dec. 1996;112(4):1703-14.
Broun P et al. Catalytic plasticity of fatty acid modification enzymes underlying chemical diversity of plant lipids. Science. Nov. 13, 1998;282(5392):1315-7.
Schena M. et al. The HAT4 gene ofArabidopsisencodes a developmental regulator. Genes Dev. 1993 7: 367-379.

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