Genes involved in polyketide synthase pathways and uses thereof

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C435S041000, C435S069100, C435S252300, C435S320100

Reexamination Certificate

active

10619532

ABSTRACT:
The subject invention relates to isolated nucleic acid sequences or genes involved in polyketide synthase (PKS) biosynthetic pathways. In particular, such pathways are involved in the production of polyunsaturated fatty acids (PUFAs) such as, for example, Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA). Specifically, the invention relates to isolating nucleic acid sequences encoding proteins involved in eukaryotic PUFA-PKS systems and to uses of these genes and encoded proteins in PUFA-PKS systems, in heterologous hosts, for the production of PUFAs such as EPA and DHA.

REFERENCES:
patent: 6140486 (2000-10-01), Facciotti et al.
patent: 6566583 (2003-05-01), Facciotti et al.
patent: 2002/0194641 (2002-12-01), Metz et al.
patent: 2004/0235127 (2004-11-01), Metz et al.
Allen, E.E., et al., “Monounsaturated but Not Polyunsaturated Fatty Acids Are Required for Growth of the Deep-Sea BacteriumPhotobacterium profundumSS9 at High Pressure and Low Temperature”,Appl. And Env. Microbiol., 65(4):1710-1720 (1999).
Kendrick, A. & Ratledge, C., “Lipids of Selected Molds Grown for Production of n-3 and n-6 Polyunsaturated Fatty Acids”,LIPIDS, 27(1):15-20 (1992).
Knutzon, D.S., et al., “Identification ofΔ5-Desaturase fromMortierella alpinaby Heterologous Expression in Bakers' Yeast and Canola”,J. Biol. Chem., 273(45):29360-29366 (1998).
Huang, Y-S., et al., “Cloning ofΔ12- andΔ6-Desaturases fromMortierella alpinaand Recombinant Production of γ-Linolenic Acid inSaccharomyces cerevisiae”, Lipids, 34(7):649-659 (1999).
Metz, J.G., “Production of Polyunsaturated Fatty Acids by Polyketide Synthases in Both Prokaryotes and Eukaryotes”,Science, 293:290-293 (2001).
Morita, N., et al., “Cloning and sequencing of clustered genes involved in fatty acid biosynthesis from the docosahexaenoic acid-producting bacterium,Vibrio marinusstrain MP-1”,Biotechnology Letters, 21:641-646 (1999).
Parker-Barnes, J. M., et al., “Identification and characterization of an enzyme involved in the elongation of n-6 and n-3 polyunsaturated fatty acids”,PNAS, 97(15):8284-8289 (2000).
Qiu, X., et al., “Identification of aΔ4 Fatty Acid Desaturase fromThraustochytriumsp. Involved in the Biosynthesis of Docosahexanoic Acid by Heterologous Expression inSaccharomyces cerevisiaeandBrassica juncea”, J. Biol. Chem., 276(34):31561-31566 (2001).
Tanaka, M., et al., “Isolation of clustered genes that are notably homologous to the eicosapentaenoic acid biosynthesis gene cluster from the docosahexaenoic acid-producing bacteriumVibrio marinusstrain MP-1”,Biotechnol. Ltrs., 21:939-945 (1999).
Watanabe, K., et al., “Fatty Acid Synthesis of an Eicosapentaenoic Acid-Producing Bacterium:De NovoSynthesis, Chain Elongation, and Desaturation Systems”,J. Biochem., 122(2):467-473 (1997).
Yazawa, K., “Production of Eicosapentaenoic Acid from Marine Bacteria”,Lipids, 31(Suppl):S297-S300 (1996).

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