Genes for diagnosing colorectal cancer

Details Genes for diagnosing colorectal cancer Genes for diagnosing colorectal cancer

2004-02-26

2009-08-18

Wessendorf, T. D. (Department: 1639)

Chemistry: analytical and immunological testing

Cancer

C435S006120, C435S004000, C424S009351, C424S009100

Reexamination Certificate

active

07575928

ABSTRACT:
This invention relates to provide the genes for diagnosing colorectal cancer, the gene sequences searching comprise the steps of: (1) deriving epithelium cells from normal intestines, polypus of intestines and colorectal cancer tissue; (2) collecting genes with highly differential gene expression by Suppression Subtractive Hybridization (SSH), and building library; (3) deriving colonies with relatively high signal intensities from cancer tissue; (4) collecting more clinically cancer tissues by Northern Hybridization, real-time Polymerase Chain Reaction (PCR) combined with analysis of bioinformation to affirm variation between differential gene expression; and (5) selecting the most suitable genes from said library, and using the gene sequence as reagent provides the effects of early diagnosis, specificity, highly sensitivity and safety.

REFERENCES:
patent: 2004/0146921 (2004-07-01), Eveleigh et al.
patent: 2005/0287544 (2005-12-01), Bertucci et al.
patent: 00/55351 (2000-09-01), None

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