Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2006-09-05
2006-09-05
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C536S023200, C435S320100, C435S069100, C435S252300, C435S348000, C435S254200, C435S254110, C435S252330, C435S325000, C530S350000
Reexamination Certificate
active
07101694
ABSTRACT:
The present invention relates to:an isolated or synthesized gene, which encodes a protein comprising the amino acid sequence represented by SEQ ID NO: 2,an isolated or synthesized gene, which encodes a protein comprising an amino acid sequence comprising a deletion, substitution or addition of one or more amino acids with respect to the amino acid sequence represented by SEQ ID NO: 2 and being capable of regenerating luciferin,an isolated or synthesized gene, which hybridizes with the complementary strand sequence of a DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 under stringent conditions and encodes a protein capable of regenerating luciferin,a recombinant DNA, which is characterized in that the above-described isolated or synthesized gene is inserted into a vector DNA,a transformant or transductant comprising the above-described recombinant DNA, anda process for producing a protein capable of regenerating luciferin, which is characterized in that the method comprises culturing the above-described transformant or transductant in a medium and collecting therefrom the protein capable of regenerating luciferin.
REFERENCES:
patent: 5814504 (1998-09-01), Kajiyama
patent: 01/25426 (2001-04-01), None
K. Okada, et al., Tetrahedron Letters, Pergamon Press, No. 32, XP-002109172, pp. 2771-2774, “Firefly Bioluminescence III. Conversion of Oxyluciferin to Luciferin in Firefly”, 1974.
K. Gomi, et al., Gene An International Journal on Genes and Genomes, ELSEVIER, vol. 294, No. 1-2, XP-004381348, pp. 157-166, “Molecular Cloning and Expression of the cDNAs Encoding Luciferinregenerating Enzyme from Luciola Cruciata and Luciola Lateralis”, Jul. 10, 2002.
Hirokawa Kozo
Kajiyama Naoki
Kurosawa Keiko
Kikkoman Corporation
Oblon & Spivak, McClelland, Maier & Neustadt P.C.
Prouty Rebecca E.
Walicka Malgorzata A.
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