Genes encoding p-coumarate 3-hydroxylase (C3H) and methods...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide encodes an inhibitory rna molecule

Reexamination Certificate

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C800S278000, C800S285000, C800S298000

Reexamination Certificate

active

07071376

ABSTRACT:
The present invention is directed to a method for altering secondary metabolism in plants, specifically phenylpropanoid metabolism. The present invention is further directed to a mutant p-coumarate 3-hydroxylase gene, referred to herein as the ref8 gene, its protein product which can be used to prepare gene constructs and transgenic plants. The gene constructs and transgenic plants are further aspects of the present invention.

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patent: 6252135 (2001-06-01), Chiang et al.
patent: WO 99/31243 (1999-06-01), None
Hu et al., Nature Biotechnology, 1999, 17:808-812.
Franke R. et al., Plant Journal 2002, vol. 30, No. 1; pp. 33-45.
Siminszky B. et al. PNAS, Feb. 1999, vol. 96, pp. 1750-1755.
Ruegger, M., et al., “Regulation of Ferulate-5-Hydroxylase Expression inArabidopsisin the Context of Sinapate Ester Biosynthesis,” Plant Physiology, Jan., 1999, 119:101-110.
Schoch, G., et al., “CYP98A3 fromArabidopsis thalianais a 3′-hydroxylase of phenolic esters, a missing link in the phenylpropanoid pathway,” J. Biol. Chem., Sep. 28, 2001, 276(39):36566-74 (manuscript attached, total of 39 pages).

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