Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Utility Patent
1995-03-14
2001-01-02
Mosher, Mary E. (Department: 1643)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
C435S320100, C435S252330, C435S252340, C536S024100, C536S023200, C536S023700
Utility Patent
active
06168945
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to DNA coding for branched chain keto dehydrogenase operon and promoter, and methods of use.
SUMMARY OF THE INVENTION
The present invention comprises an isolated double stranded DNA sequence encoding a polypeptide possessing activity of branched chain keto acid dehydrogenase, and the polypeptide encoded by this sequence. The activity of the branched chain keto acid dehydrogenase is to catalyze the oxidation of branched chain keto acids. Also, the present invention comprises an isolated double stranded DNA sequence useful as a promoter of gene expression in the genus Pseudomonas or Escherichia.
The present invention further comprises a bacterium from the genus Escherichia comprising at least one recombinant vector comprising a gene encoding branched chain keto acid dehydrogenase, and a bacterium from the genus Pseudomonas comprising at least one recombinant vector comprising a promoter of gene expression having the characteristics of the DNA sequence of ATCC 88403.
The present invention further comprises a recombinant expression vector comprising a DNA sequence encoding branched chain keto acid dehydrogenase. The vector is capable of expressing branched chain keto acid dehydrogenase in a transformed microorganism or cell culture. Another recombinant expression vector comprises the DNA sequence of a promoter of gene expression in the genus Pseudomonas or Escherichia having the characteristics of the DNA sequence of ATCC 88403.
Additionally, the present invention comprises a method of producing the polypeptide branched chain keto acid dehydrogenase comprising isolating the gene encoding branched chain keto acid dehydrogenase. The isolated gene is disposed in a vector capable of permitting expression of the gene. The recombinant vector is then disposed in a host capable of permitting expression of the gene and the gene expressed.
REFERENCES:
patent: 5656483 (1997-08-01), Sokatch et al.
Sykes et al., Apr. 1985, Journal Of Bacteriology, vol. 162, pp. 203-208, Conjugative Mapping Of Pyruvate, 2-Ketoglutarate, and Branched-Chain Keto Acid Dehydrogenase Genes inPseudomonas putidaMutants.
Luthi et al., 1986, Journal of general Microbiology, vol. 132, pp. 2667-2675, The arcABC Operon Required for Fermentative Growth ofPseudomonas aeruginosaon Arginine: Tn5-751-assisted Cloning and Localization of Structural Genes.
Inouye et al., Jun. 1986, Journal of Bacteriology, vol. 166, pp. 739-745, Nucleotide Sequence of a DNA Segment Promoting Transcription inPseudomonas putida.
Minton et al., Dec. 1983, Journal of Bacteriology, vol. 156, pp. 1222-1227, Molecular Cloning of the Pseudomonas Carboxypeptidase G2Gene and Its Expression inEscherichia coliandPseudomonas putida.
Schell, Feb. 1983, Journal of Bacteriology, vol. 153, pp. 822-829, Cloning and Expression inEscherichia coliof the Naphthalene Degradation Genes from Plasmid NAH7.
Madhusudhan et al., Oct. 1990, Journal of Bacteriology, vol. 172, pp. 5655-5663, Transcriptional Analysis of the Promoter region of thePseudomonas putidaBranched-Chain Keto Acid Dehydrogenase Operon.
Sokatch et al., Nov. 1981, Journal of Bacteriology, vol. 148, pp. 639-646, Isolation of a Specific Lipoamide Dehydrogenase for a Branched-Chain Keto Acid Dehydrogenase fromPseudomonas putida.
Burns et al., Feb. 1988, Eur. Journal of Biochemistry, vol. 176, pp. 165-169, Comparison of the amino acid sequences of the transacylase components of branched chain oxoacid dehydrogenase ofPseudomonas putidaand the pyruvate and 2-oxoglutarate dehydrogenases ofEscherichia coli.
Sykes et al., Apr. 1987, Journal of Bacteriology, vol. 169, pp. 1619-1625, Molecular Cloning of Genes Encoding Branched-Chain Keto Acid Dehydrogenase ofPseudomonas putida.
Burns et al., Feb. 1988, Eur. Journal of Biochemistry, vol. 176, pp. 165-169, Comparison of the amino acid sequences of the transacylase components of branched chain oxoacid dehydrogenase ofPseudomonas putidaand the pyruvate and 2-oxoglutarate dehydrogenases ofEscherichia coli.
Yanisch-Perroc et al., 1985, Gene, vol. 33, pp. 103-119, Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 andpUC19 vectors.
Sokatch et al., Nov. 1981, Journal of Bacteriology, vol. 148, pp. 647-652, Purification of a Branched-Chain Keto Acid Dehydrogenase fromPseodomonas putida.
Bahdasarian et al., 1981, Gene, vol. 16, pp. 237-247, Specific-purpose plasmid cloning vectors.
Madhusudhan K. T.
Oh Soo-Kyung
Sokatch John R.
Sykes Pamela J.
You Ji Chang
Dunlap Codding & Rogers P.C.
Mosher Mary E.
The Board of Regents of the University of Oklahoma
You Ji Chang
LandOfFree
Genes encoding operon and promoter for branched chain keto... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Genes encoding operon and promoter for branched chain keto..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Genes encoding operon and promoter for branched chain keto... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2510370