Genes encoding novel proteolytic enzymes

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S320100, C435S254300, C435S212000, C435S252300, C536S023200

Reexamination Certificate

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10469204

ABSTRACT:
The invention relates to newly identified gene sequences that encode novel proteases obtainable fromAspergillus niger. The invention features the full length gene sequence of the novel genes, their cDNA sequences as well as the full-length functional protein and fragments thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with DNA according to the invention and cells wherein a protease according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.

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patent: 02/68623 (2002-12-01), None
patent: 02/45523 (2003-01-01), None
International Preliminary Examination Report for PCT/EP02/01984, completed on Jul. 18, 2003, 9 pages.
International Search Report for PCT/EP02/01984, mailed on Sep. 18, 2002, 3 pages.
Jarai et al., Current Genetics (1994) 26:238-244.
U36471, EMBL, EBI, “Neurospora crassa vacuolar protease A (pep-4) gene, complete cds” (Oct. 25, 1995).
Van Den Hombergh et al., Gene (1994) 151:73-79.

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