Genes encoding branched-chain alpha-ketoacid dehydrogenase compl

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...

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536 71, 4352535, 435 76, 514 30, C12P 1718, C07H 1708

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057078394

ABSTRACT:
The present invention relates to novel DNA sequences that encode for the branched-chain alpha-ketoacid dehydrogenase complex of an organism belonging to the genus Streptomyces and to novel polypeptides produced by the expression of such sequences. It also relates to novel methods of enhancing the production of natural avermectin, of producing a Streptomyces avermitilis bkd mutant and of producing novel avermectins through fermentation.

REFERENCES:
patent: 5077278 (1991-12-01), Hafner et al.
patent: 5252474 (1993-10-01), Gewain et al.
Sykes et al, "Molecular Cloning of Genes Encoding Branched-Chain Keto Acid Dehydrogenase of Pseudomonas putida", J. Bacteriol. (1987)169: 1619-1625.

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