Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase
Reexamination Certificate
2005-03-01
2005-03-01
Achutamurthy, Ponnathapura (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Lyase
C435S183000, C435S320100, C536S023200
Reexamination Certificate
active
06861243
ABSTRACT:
New genes containing a DNA sequence coding for hydroxynitrile lyase, which genes can be prepared via a primer combination based on the DNA sequence of the 5′-region of the mdl genes fromPrunus serotinaand fromPrunus amygdalusand/or a primer 2 based on the 3′-region of the DNA sequences of one of the hydroxynitrile lyase isoenzymes fromPrunus serotinaor fromPrunus amygdalus, subsequent amplification with a DNA polymerase using a DNA from organisms, containing genes coding for hydroxynitrile lyase, as templates and cloning, and also recombinant proteins which can be prepared in suitable host cells by heterologous expression of the DNA sequence of said HNL genes, and proteins and fusion proteins derived therefrom and use of said proteins for preparing (R)- or (S)-cyanohydrins.
REFERENCES:
patent: 6046042 (2000-04-01), Hasslacher et al.
Attwood et al. Which craft is best in bioinformatics? Comput. Chem. 2001, vol. 25(4), pp. 329□339.*
Ponting, C.P.Issues in predicting protein function from sequence. Brief. Bioinform. Mar. 2001, vol. 2(1), pp. 19□29.*
Hu et al. Accession U51562. Apr. 11, 1996.*
ExPASY NiceZyme View of ENZYME: EC 4.1.2.10 Mandelonitrile lyase.*
Suelves et al., Planta, vol. 206, pp. 388-393 (1998).
Hu et al., Plant Physiology, vol. 119, pp. 1535-1546 (1999).
Bona Rodolfo
Dreveny Ingrid
Glieder Anton
Kratky Christoph
Mayrhofer Herbert
Achutamurthy Ponnathapura
DSM Fine Chemicals Austria NFG GmbH & Co. KG
Fronda Christian L.
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