Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide encodes an inhibitory rna molecule
Reexamination Certificate
2007-03-08
2010-06-08
Grunberg, Anne Marie (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide encodes an inhibitory rna molecule
C800S278000, C800S295000, C800S298000, C435S320100, C536S023100
Reexamination Certificate
active
07732664
ABSTRACT:
Modern sugarcane cultivars are complex hybrids resulting from crosses among several species of theSaccharumgenus. Traditional breeding methods have been extensively employed in different countries along the past decades to develop varieties with increased sucrose yield, and resistant to plagues and diseases. Conventional varietal improvement is, however, limited by the narrow pool of suitable markers. In this sense, molecular genetics is seen as a promising tool to assist in the process of molecular marker identification. The present invention concerns the identification of 348 genes associated with sucrose content in sugarcane plants. The genes were found to be differentially expressed when high sucrose and low sucrose plants and populations of plants were compared and/or when high and low sucrose internodes were compared. The expression data was obtained using cDNA microarray and quantitative PCR technologies. The genes identified can be used to identify, distinguish, characterize and/or develop plants with increased sucrose content. More preferably SEQ ID Nos: 1 to 203 should be useful as molecular markers. SEQ ID Nos: 204 to 228 are given as controls or examples of genes never associated with sucrose content. SEQ ID Nos. 1-203 and SEQ ID Nos. 229 to 373 can be targeted in the development of transgenic or non-transgenic varieties with increased sucrose content.
REFERENCES:
Colliver et al 1997 Plant Molecular Biology 35:509-522.
International Search Report mailed Sep. 30, 2008, for PCT Application No. PCT/BR07/00282 filed Aug. 22, 2007, 4 pages.
Papini-Terzi, F.S. et al. (2005). “Transcription Profiling of Signal Transduction-related Genes in Sugarcane Tissues,”DNA Research12:27-38.
Rolland, F. et al. (2002). “Sugar Sensing and Signaling in Plants,”Plant Cell14:S185-S205.
Vettore, AL. et al. (2003). “Analysis and Functional Annotation of an Expressed Sequence Tag Collection for Tropical Sugarcane,”Genome Research13:2725-2735.
Buckeridge Marcos
de Maria Felix Juliana
Marques Josélia Oliveira
Papini-Terzi Flavia Stal
Pereira de Souza Amanda
Central de Alcool Lucelia Ltda.
Centro de Tecnologia Canavieira
Fundacao de Amparo a Pesquisa do Estado de Sao Paulo - Fapesp.
Grunberg Anne Marie
Morrison & Foerster / LLP
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