Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1993-10-26
1996-12-24
Woodward, Michael P.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 723, 435 724, 435974, 436543, C12Q 170, G01N 33574, G01N 33567, G01N 33564
Patent
active
055872859
ABSTRACT:
Disclosed is a highly sensitive anti-HIV antibody detection assay. The assay detects the presence of anti-HIV antibodies through the use of a non-denatured HIV antigenic determinant which immunoreactivity binds anti-HIV antibodies in a biological sample. The non-denatured HIV antigenic determinant has provided a means for detecting anti-HIV antibodies in serum samples testing seronegative for the presence of HIV antibodies directed against denatured HIV antigens p17, p19, p24, p27, p39, gp41, p55, gp120 and gp160. The antigenic determinant may take the form of a cross-immunoreactive live HIV-infected cell line or a biologically engineered peptide possessing conserved or shared HIV surface antigenic determinants. Methods of preparing HIV target antigens are also disclosed, as well as methods for determining the presence of anti-HIV antibodies through the use of these non-denatured HIV target antigens. A test kit for detecting anti-HIV antibodies is also disclosed.
REFERENCES:
patent: 4357310 (1982-11-01), Chan et al.
patent: 4722888 (1988-02-01), Broder
patent: 4725669 (1988-02-01), Essex
Groupmon et al, Blood, 66(3):742-44. 1985.
Salahudin et al, Lancet 12 22-291 84. pp. 1418-1420. 1984.
Lee et al, PNAS 81:3856-60 1984.
Homma et al, Seienu 225:716-718 1984.
Tijssew, "Practice & Theory Enzyme Immunoassay", Elsevier 1985, NY pp. 329-332.
Harlow, "Antibodies, A Laboratory Manual", Cold Spring Harbor Press, NY. 1988. p. 184.
Kitchen et al, Nature 312:367-369, 1984.
Essex et al, Science 221: 1061-1064, 1883, (Essex "a").
Essex et al, Science 220:854-862, 1983 (Essex "b").
Gallo et al. (1984), Science, 244:500-3.
Saah et al. (1986), J. Clin. Microbiol., 25(a):1605.
Chou et al. (1988), J. Infect. Disease, 157(4):805-11.
Barin et al. (1985), Science, 228;1094-6.
Kitchen et al. (1986), J. Infect. Dis., 153:788-90.
Ranki et al. (1987), Lancet, 2:589-93.
Imagawa et al. (1989), New J. Med., 320(22):1458-89.
Weiss, R. (Jun. 3, 1989), Science News, 135:340.
Dupont Biotecl Update (May 1989), "Improved HIV p24 Core Profile ELISA: NEK-060, NEK-060A".
Allain et al. (1986), Lancet, 1233:35.
Goudsmit et al. (1987), J. Inf. Disease, 155(3):558-60.
Franchini et al. (1987), Blood, 69(2):437-41.
Sarngadharen et al. (1984), Science, 224:506-8.
Hofbauer et al. (1988), J. Clin. Micro., 26(1):116-20.
Ujhelyi et al. (1987), AIDS, 1:161-5.
Shepp et al. (1987), AIDS, 2:113-7.
Lange et al. (1987), AIDS, 1:15-20.
Ferroni et al. (1988), Vox Sang, 55:143-7.
Harada et al. (1987), Virology, 158:447-51.
Huisma et al. (1987), Vox Sang, 53:31-6.
DiMarzo et al. (1985), Proc. Natl. Acad. Sci., 82:5199-02.
Lasky et al. (1986), Science, 233:209-33.
Cloyd et al. (1977), J. Clin. Microb., 5(1):86-90.
Cloyd et al. (1987), Virology, 161:286-92.
Barre-Sinoussi et al. (1983), Science, 220:868-71.
Popovich et al. (1984), Science, 224:497-500.
Coffin et al. (1986), Nature, 321:10.
Auerbach et al. (1982), N.J.M., 306:248-52.
Burke et al. (1986), JAMA, 256:347.
Salahuddin et al. (1984), Lancet, 2:1418-20.
Mayer et al. (1986), Ann, Intern. Med., 104:194-6.
Groopman et al. (1985), Blood, 66:742-4.
Wolinsky et al. (1988), Fourth International Conference on Aids, Stockholm Abstract No. 1099:137.
Loche et al. (1988), Lancet, 2:418-21.
Miles et al. (1986), Nature, 219:186.
Butler et al. (1978), Immunochemistry, 15:131-6.
Haseltine, W. W. (1989), N. Eng. J. Med., 320:1487-9.
Jemmerson et al. (1986), Science, 232:1001-4.
Poiesz et al. (1980), Proc. Natl. Acad. Sci., USA, 77:7415.
Robert-Guroff et al. (1985), Nature, 316:72-4.
Cloyd et al. (1979), J. Exp. Med., 149:702-12.
Laemmli (1970), Nature, 227:680-5.
Robey et al. (1986), Proc. Natl. Acad. Sci., USA, 84:7023-7.
R. Haschemeyer and A. HAschemeyer, eds (1973) In: Proteins, A Guide to Study By Physical and Chemical Methods, Chapter XV:352-385.
Tanford, C. and Reynolds, J. A., "Characterization of Membrane Proteins in Detergent Solutions, " Biochimica et Biophysica Acta, 457:133-170 (1976).
Helenius, A., et al., "Properties of Detergenets," Methods in Enzymology, LVI: 734-749 (1979).
Hjelmeland, L. M., and Chrambach, A., "electrophoresis and electrofocusing in detergent containing media: A discussion of basic concepts," Electrophoresis, 2:1-11 (1981).
Chassagne et al., "A Monoclonal Antibody Against Lav GAG Precursor: use For Viral Protein Analysis and Antigenic Expression In Infected Cells," The Journal of Immunology, 136(4):1442-1445, 1989.
Chiodi et al., "Site-Directed ELISA With Synthetic Peptides Representing the HIV Transmembrane glycoprotein," Journal of Medical Virology, 23:1-9, 1987.
Clerici et al., "Eavesdropping on Rare `Silent` HIV-1 Infection," The Journal of NIH Research, 3:71-76, 1991.
Cohen et al., "Identification of a Protein Encoded by the vpu Gene of HIV-1," Nature, 334:532-534. 1988.
D uzg un et al., "Der Nachweis von HIV-1-Antigen--Bestimmung mit Hilfe von drei verschiedenen Testsystemen," Klin Wochenschr, 66:212-215, 1988.
Evans et al., "Clinical evaluationof Abbott and Wellcome enzyme linked immunosorbent assays for detection of serum antibodies to human immunodeficiency virus (HIV)," J. Clin. Pathol., 40:552-555, 1987.
Genesca et al., "Comparison of Four Different Antigen-Capture Assays for the Detection of Human Immunodeficiency Virus Antigen (HIV-Ag)," Journal of Acquired Immune Deficiency Syndromes, 2:170-177, 1989.
Hood et al., "," Immunology, ?
Houn et al., "Status of Current Clinical Tests for Human Immunodeficiency Virus (HIV: Applications and Limitations," Annals. of Clinical and Laboratory Science, 17(5):279-285, 1987.
Mervis et al., "the gag Gene Products of Human Immunodeficiency Virus Type 1: Alignment within the gag Open Reading Frame, Identification of Posttranslational Modifications, and Evidence for Alternative gag Precursors," Journal of Virologly, 62 (11):3993-4002, 1988.
Nishanian et al., "Significance of Quantitative Enzyme-Linked Immunosorbent Assay (ELISA) Results in Evaluation of Three ELISAs and Western Blot Tests for Detection of Antibodies to Human Immunodeficiency Virus in a High-Rist Population," Journal of Clinical Microbiology, 25(2):395-400, 1987.
Pal et al., "Processing of the structural proteins of human immunodeficiency virus type 1 in the presence of monensin and cerulenin," Proc. Natl. Acad. Sci. USA, 85:9283-9286, 1988.
Race et al., "Human Immunodeficiency Virus Infection Elicits Early Antibody Not Detected by Standard Tests: Implications for Diagnostics and Viral Immunololgy," Virology, 184:001-007, 1991.
Rey et al., "Characterization of Human Immunodeficiency Virus Type 2 EnvelopeGlycoproteins: Dimerization of the Glycoprotein precursor during Processing," Journal of Virology, 63(2):647-658, 1989.
Sligh et al., "Flow Cytometric Indirect Immunofluorescence Assay with High Sensitivity and Specificity for Detection of Antibodies to Human Immunodeficiency Virus (HIV)," A.J.C.P., 91(2):210-214, 1989.
Steckelberg et al., "Serologic Testing for Human Immunodeficiency Virus Antibodies," Mayo Clin. Proc., 63:373-380, 1988.
Cloyd Miles W.
Ramsey Keith M.
Mayfield Denise L.
University of Texas System
Woodward Michael P.
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