Generation of xenogeneic antibodies

Details Generation of xenogeneic antibodies Generation of xenogeneic antibodies

1995-06-05

2000-09-05

Stanton, Brian R.

Multicellular living organisms and unmodified parts thereof and

Nonhuman animal

Transgenic nonhuman animal

800 18, 800 3, 800 4, 800 22, 800 25, 435325, 435354, C12N 510, C12N 1579, A61K 4800

Patent

active

06114598&

ABSTRACT:
The subject invention provides non-human mammalian hosts characterized by inactivated endogenous Ig loci and functional human Ig loci for response to an immunogen to produce human antibodies or analogs thereof. The hosts are produced by multiple genetic modifications of embryonic cells in conjunction with breeding. Different strategies are employed for recombination of the human loci randomly or at analogous host loci. Chimeric and transgenic mammals, particularly mice, are provided, having stably integrated large, xenogeneic DNA segments. The segments are introduced by fusion with yeast spheroplasts comprising yeast artificial chromosomes (YACs) which include the xenogeneic DNA segments and a selective marker such as HPRT, and embryonic stem cells.

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