Generation of single-strand circular DNA from linear...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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Reexamination Certificate

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07041480

ABSTRACT:
The present invention provides a method for the rapid simultaneous production of a plurality of single-stranded DNA circles having a predetermined size and nucleotide sequence using pre-designed hairpin oligonucleotides containing complementary sequences for directing ligation to form dumbbell-shaped monomers followed by heat denaturation to yield single-stranded DNA circles.

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Diegelman et al., “Generation of Circular RNAs and Trans-cleaving Catalytic RNAs by Rolling Transcription of Circular DNA Oligonucleotides Encoding Hairpin Ribozymes”, Nucleic Acids Research, vol. 26, No. 13, pp. 3235-3241 (1998).
Lim et al., “Synthesis of DNA Dumbbells: Chemical vs. Enzymatic Ligation of Self-Complementary Oligonucleotides”, Nucleoside and Nucleotides, 16 (1&2), pp. 41-51 (1997).
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